RRC ID 2192
Author Asakawa H, Hayashi A, Haraguchi T, Hiraoka Y.
Title Dissociation of the Nuf2-Ndc80 complex releases centromeres from the spindle-pole body during meiotic prophase in fission yeast.
Journal Mol. Biol. Cell
Abstract In the fission yeast Schizosaccharomyces pombe, centromeres remain clustered at the spindle-pole body (SPB) during mitotic interphase. In contrast, during meiotic prophase centromeres dissociate from the SPB. Here we examined the behavior of centromere proteins in living meiotic cells of S. pombe. We show that the Nuf2-Ndc80 complex proteins (Nuf2, Ndc80, Spc24, and Spc25) disappear from the centromere in meiotic prophase when the centromeres are separated from the SPB. The centromere protein Mis12 also dissociates during meiotic prophase; however, Mis6 remains throughout meiosis. When cells are induced to meiosis by inactivation of Pat1 kinase (a key negative regulator of meiosis), centromeres remain associated with the SPB during meiotic prophase. However, inactivation of Nuf2 by a mutation causes the release of centromeres from the SPB in pat1 mutant cells, suggesting that the Nuf2-Ndc80 complex connects centromeres to the SPB. We further found that removal of the Nuf2-Ndc80 complex from the centromere and centromere-SPB dissociation are caused by mating pheromone signaling. Because pat1 mutant cells also show aberrant chromosome segregation in the first meiotic division and this aberration is compensated by mating pheromone signaling, dissociation of the Nuf2-Ndc80 complex may be associated with remodeling of the kinetochore for meiotic chromosome segregation.
Volume 16(5)
Pages 2325-38
Published 2005-5
DOI 10.1091/mbc.e04-11-0996
PII E04-11-0996
PMID 15728720
PMC PMC1087238
MeSH Base Sequence Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Centromere / metabolism DNA, Fungal / genetics Genes, Fungal Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Kinetochores / metabolism Meiosis Models, Biological Nuclear Proteins / genetics Nuclear Proteins / metabolism* Peptides / metabolism Prophase Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Schizosaccharomyces / cytology* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Signal Transduction
IF 3.512
Times Cited 34
WOS Category CELL BIOLOGY
Resource
Yeast FY15688(HA252-4A) FY15689(HA525) FY15690(HA205-2A) FY15691(HA229-11A) FY15692(HA485-10D) FY15693(HA259-1D) FY15694(HA260-7D) FY15695(HA309-11C) FY15696(HA495-9B) FY15697(HA499-1C) FY15698(HAR3021-1) etc.