Reference - Detail
| RRC ID | 21954 |
|---|---|
| Author | Sundram V, Ng FS, Roberts MA, Millán C, Ewer J, Jackson FR. |
| Title | Cellular requirements for LARK in the Drosophila circadian system. |
| Journal | J Biol Rhythms |
| Abstract |
RNA-binding proteins mediate posttranscriptional functions in the circadian systems of multiple species. A conserved RNA recognition motif (RRM) protein encoded by the lark gene is postulated to serve circadian output and molecular oscillator functions in Drosophila and mammals, respectively. In no species, however, has LARK been eliminated, in vivo, to determine the consequences for circadian timing. The present study utilized RNA interference (RNAi) techniques in Drosophila to decrease LARK levels in clock neurons and other cell types in order to evaluate the circadian functions of the protein. Knockdown of LARK in timeless (TIM)- or pigment dispersing factor (PDF)-containing clock cells caused a significant number of flies to exhibit arrhythmic locomotor activity, demonstrating a requirement for the protein in pacemaker cells. There was no obvious effect on PER protein cycling in lark interference (RNAi) flies, but a knockdown within the PDF neurons was associated with increased PDF immunoreactivity at the dorsal termini of the small ventral lateral neuronal (s-LNv) projections, suggesting an effect on neuropeptide release. The expression of lark RNAi in multiple neurosecretory cell populations demonstrated that LARK is required within pacemaker and nonpacemaker cells for the manifestation of normal locomotor activity rhythms. Interestingly, decreased LARK function in the prothoracic gland (PG), a peripheral organ containing a clock required for the circadian control of eclosion, was associated with weak population eclosion rhythms or arrhythmicity. |
| Volume | 27(3) |
| Pages | 183-95 |
| Published | 2012-6-1 |
| DOI | 10.1177/0748730412440667 |
| PII | 27/3/183 |
| PMID | 22653887 |
| PMC | PMC4135585 |
| MeSH | Animals Animals, Genetically Modified Circadian Rhythm* Drosophila Proteins / metabolism* Drosophila Proteins / physiology* Female Gene Expression Regulation Locomotion Male Models, Biological Neurons / metabolism Phenotype RNA Interference RNA Processing, Post-Transcriptional RNA-Binding Proteins / metabolism RNA-Binding Proteins / physiology* |
| IF | 3.122 |
| Times Cited | 10 |
| WOS Category | BIOLOGY PHYSIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 8597R-1 8597R-2 |