RRC ID 21962
Author Neisch AL, Speck O, Stronach B, Fehon RG.
Title Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
Journal J Cell Biol
Abstract Precisely controlled growth and morphogenesis of developing epithelial tissues require coordination of multiple factors, including proliferation, adhesion, cell shape, and apoptosis. RhoA, a small GTPase, is known to control epithelial morphogenesis and integrity through its ability to regulate the cytoskeleton. In this study, we examine a less well-characterized RhoA function in cell survival. We demonstrate that the Drosophila melanogaster RhoA, Rho1, promotes apoptosis independently of Rho kinase through its effects on c-Jun NH(2)-terminal kinase (JNK) signaling. In addition, Rho1 forms a complex with Slipper (Slpr), an upstream activator of the JNK pathway. Loss of Moesin (Moe), an upstream regulator of Rho1 activity, results in increased levels of Rho1 at the plasma membrane and cortical accumulation of Slpr. Together, these results suggest that Rho1 functions at the cell cortex to regulate JNK activity and implicate Rho1 and Moe in epithelial cell survival.
Volume 189(2)
Pages 311-23
Published 2010-4-19
DOI 10.1083/jcb.200912010
PII jcb.200912010
PMID 20404112
PMC PMC2856900
MeSH Animals Animals, Genetically Modified Apoptosis / physiology* Cell Membrane / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / anatomy & histology Drosophila melanogaster / physiology Enzyme Activation Epithelial Cells / cytology Epithelial Cells / physiology JNK Mitogen-Activated Protein Kinases / genetics JNK Mitogen-Activated Protein Kinases / metabolism* MAP Kinase Kinase Kinases / genetics MAP Kinase Kinase Kinases / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism RNA Interference Signal Transduction / physiology* Wings, Animal / anatomy & histology Wings, Animal / physiology rho GTP-Binding Proteins / genetics rho GTP-Binding Proteins / metabolism*
IF 8.891
Times Cited 56
Drosophila 1388R-1 2272R-1 4353R-2 4353R-3 5680R-1