Reference - Detail
|Author||Kitajima TS, Sakuno T, Ishiguro K, Iemura S, Natsume T, Kawashima SA, Watanabe Y.|
|Title||Shugoshin collaborates with protein phosphatase 2A to protect cohesin.|
Sister chromatid cohesion, mediated by a complex called cohesin, is crucial--particularly at centromeres--for proper chromosome segregation in mitosis and meiosis. In animal mitotic cells, phosphorylation of cohesin promotes its dissociation from chromosomes, but centromeric cohesin is protected by shugoshin until kinetochores are properly captured by the spindle microtubules. However, the mechanism of shugoshin-dependent protection of cohesin is unknown. Here we find a specific subtype of serine/threonine protein phosphatase 2A (PP2A) associating with human shugoshin. PP2A colocalizes with shugoshin at centromeres and is required for centromeric protection. Purified shugoshin complex has an ability to reverse the phosphorylation of cohesin in vitro, suggesting that dephosphorylation of cohesin is the mechanism of protection at centromeres. Meiotic shugoshin of fission yeast also associates with PP2A, with both proteins collaboratively protecting Rec8-containing cohesin at centromeres. Thus, we have revealed a conserved mechanism of centromeric protection of eukaryotic chromosomes in mitosis and meiosis.
|MeSH||Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Centromere / metabolism* Chromatids / metabolism* Chromosomal Proteins, Non-Histone / metabolism* Chromosome Pairing* HeLa Cells Humans Meiosis Mitosis Multiprotein Complexes / genetics Multiprotein Complexes / metabolism Nuclear Proteins / metabolism* Phosphoprotein Phosphatases / classification Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism* Phosphoproteins / metabolism Phosphorylation Protein Binding Protein Phosphatase 2 Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / metabolism|
|WOS Category||CELL BIOLOGY|
|Yeast||FY13797(PZ985) FY13798(PZ986) FY13777(PZ688) FY13779(PZ690) FY13780(PZ694) FY13778(PZ689) FY13765(PZ164)|