RRC ID 21996
著者 Zartman J, Restrepo S, Basler K.
タイトル A high-throughput template for optimizing Drosophila organ culture with response-surface methods.
ジャーナル Development
Abstract The Drosophila wing imaginal disc is a key model organ for molecular developmental genetics. Wing disc studies are generally restricted to end-point analyses of fixed tissues. Recently several studies have relied on limited data from discs cultured in uncharacterized conditions. Systematic efforts towards developing Drosophila organ culture techniques are becoming crucial for further progress. Here, we have designed a multi-tiered, high-throughput pipeline that employs design-of-experiment methods to design a culture medium for wing discs. The resulting formula sustains high levels of proliferation for more than 12 hours. This approach results in a statistical model of proliferation as a function of extrinsic growth supplements and identifies synergies that improve insulin-stimulated growth. A more dynamic view of organogenesis emerges from the optimized culture system that highlights important facets of growth: spatiotemporal clustering of cell divisions and cell junction rearrangements. The same approach could be used to improve culture conditions for other organ systems.
巻・号 140(3)
ページ 667-74
公開日 2013-2-1
DOI 10.1242/dev.088872
PII 140/3/667
PMID 23293298
MeSH Animals Cell Culture Techniques / methods* Cell Culture Techniques / standards Cell Division Cell Line Cell Proliferation Culture Media / metabolism Culture Media / standards* Drosophila / cytology* Drosophila / metabolism High-Throughput Screening Assays / methods High-Throughput Screening Assays / standards Imaginal Discs / cytology* Imaginal Discs / metabolism Insulin / metabolism Larva / cytology Larva / metabolism Organ Culture Techniques / methods Organ Culture Techniques / standards Serum / metabolism Time-Lapse Imaging Wings, Animal / cytology Wings, Animal / metabolism
IF 5.611
引用数 45
WOS 分野 DEVELOPMENTAL BIOLOGY
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