Reference - Detail
| RRC ID | 22008 |
|---|---|
| Author | Kimura S. |
| Title | The Nap family proteins, CG5017/Hanabi and Nap1, are essential for Drosophila spermiogenesis. |
| Journal | FEBS Lett |
| Abstract |
Spermiogenesis is a dynamic process leading to alterations in cell morphology. In spermiogenesis, the roles of the histone chaperones are largely unknown. Here, I report the unexpected roles of two Nap family proteins, CG5017/Hanabi and nucleosome assembly protein 1 (Nap1) in Drosophila. Hanabi is mainly localized in the cytoplasm, and the hanabi mutant shows fully scattered nuclei and abnormality of nuclear shaping in spermatid elongation. In contrast, Nap1 is localized at the apical tip of the sperm head, and the nap1 mutant exhibits disruption of the nuclear bundle in the later stage. These findings imply that Nap family proteins might individually sustain cytoskeleton-based morphogenesis, rather than histone biogenesis. |
| Volume | 587(7) |
| Pages | 922-9 |
| Published | 2013-4-2 |
| DOI | 10.1016/j.febslet.2013.02.019 |
| PII | S0014-5793(13)00150-6 |
| PMID | 23454210 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Blotting, Western Cell Nucleus / metabolism Cytoplasm / metabolism Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Gene Expression Genes, Essential / genetics Luminescent Proteins / genetics Luminescent Proteins / metabolism Male Microscopy, Phase-Contrast Molecular Sequence Data Mutation Nucleosome Assembly Protein 1 / genetics* Nucleosome Assembly Protein 1 / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Sperm Head / metabolism Spermatogenesis / genetics* Testis / metabolism |
| IF | 3.057 |
| Times Cited | 13 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Drosophila | GS10858 (DGRC#202874) DGRC#109815 |