RRC ID 22010
著者 Ly LL, Suyari O, Yoshioka Y, Tue NT, Yoshida H, Yamaguchi M.
タイトル dNF-YB plays dual roles in cell death and cell differentiation during Drosophila eye development.
ジャーナル Gene
Abstract Nuclear transcription factor Y (NF-Y) is well characterized in eukaryotes. It consists of three different subunits, NF-YA, NF-YB and NF-YC, all of which are required for formation of the NF-Y complex and DNA-binding. There is a high homology in NF-YB among Drosophila species with 75% identity and 95% similarity overall, especially in the histone-fold motif (HFM) (95% identity and 100% similarity). In the present study, specific knockdown of Drosophila NF-YB (dNF-YB) in eye imaginal discs induced a rough eye phenotype in adults and this phenotype was the result of induction of caspase-dependent apoptosis followed by apoptosis-induced proliferation. Furthermore, knockdown specifically inhibited R7 photoreceptor cell differentiation, independent of the apoptotic function. dNF-YB and dNF-YA indeed form complexes in vivo where they impair R7 photoreceptor cell differentiation by down regulating the mitogen-activated protein kinase (MAPK) pathway. Expression of the sev gene, or the D-raf gene, a downstream component of the MAPK cascade, could rescue the rough eye phenotype and the loss of R7 signals in dNF-YB knockdown flies. The death executioner Bcl-2 (debcl) is the homolog of Bcl-2 in Drosophila melanogaster and its promoter contains four dNF-Y-binding consensus sequences which play positive roles in promoter activity. In chromatin immunoprecipitation assays with anti-dNF-YB antibody and S2 cells, the debcl gene promoter region containing the NF-Y consensus was effectively amplified in immunoprecipitates by polymerase chain reaction. Taken together, these results indicate that dNF-Y regulates debcl gene expression.
巻・号 520(2)
ページ 106-18
公開日 2013-3-9
DOI 10.1016/j.gene.2013.02.036
PII S0378-1119(13)00226-6
PMID 23470843
MeSH Amino Acid Sequence Animals Animals, Genetically Modified CCAAT-Binding Factor / genetics CCAAT-Binding Factor / physiology* Cell Death / genetics Cell Differentiation / genetics* Cell Line Drosophila melanogaster / embryology* Drosophila melanogaster / genetics Embryo, Nonmammalian Eye / embryology* Eye / metabolism Gene Expression Regulation, Developmental Gene Knockdown Techniques Molecular Sequence Data Proto-Oncogene Proteins c-bcl-2 / genetics Sequence Homology Transfection
IF 2.415
引用数 11