論文 - 詳細
| RRC ID | 2220 |
|---|---|
| 著者 | Nishida Y, Ono B. |
| タイトル | An experimental system for the study of mutations in the HMR locus of Saccharomyces cerevisiae: the insertion of Ty into HMRa vs. the conversion of HMRa to HMRalpha. |
| ジャーナル | Yeast |
| Abstract |
A cross between a sir4-11 strain (sir4-11 HMLalpha MATalpha HMRa, non-mating type) and an a-mating strain (SIR(+) HMLalpha MATa HMRa) of Saccharomyces cerevisiae forms diploid clones at a frequency of 5 x 10(-6), but the obtained diploid clones often (>70%) have altered forms of the HMRa-containing restriction fragment, designated @ HMRa'. We previously found that some HMRa's are associated with the conversion of HMRa to HMRalpha. In this report, we present evidence that another @ HMRa' associates with the insertion of Ty into Ya of HMR. We also found that the sir4-11 strain increased mating frequency by UV irradiation to a level of 9 x 10(-4), and that generation of HMRa' was completely prevented by disruption of RAD52 of the sir4-11 strain. Hence, we conclude that the mutations that cause generation of HMRa' occur in the sir4-11 strain prior to mating. Due to these mutations, the sir4-11 strain converts to alpha-mating type and readily mates with the a-mating strain. We discuss the usefulness of the sir4-11 strain for the study of mutations in the HMR locus of S. cerevisiae. |
| 巻・号 | 24(9) |
| ページ | 723-30 |
| 公開日 | 2007-9-1 |
| DOI | 10.1002/yea.1507 |
| PMID | 17566140 |
| MeSH | Chromosome Walking DNA, Fungal / genetics Genes, Mating Type, Fungal / genetics* Molecular Sequence Data Mutagenesis, Insertional Mutation Polymerase Chain Reaction Retroelements / genetics* Saccharomyces cerevisiae / genetics* Sequence Analysis, DNA Silent Information Regulator Proteins, Saccharomyces cerevisiae / genetics* |
| IF | 3.143 |
| 引用数 | 1 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MYCOLOGY MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 酵母 | BY25348(RF152-18A) BY25350(IS361-11D) BY25351(IS361r) BY25354(RI2) BY25355(RI2-1C) BY25352(RI1) BY25353(RI1-2C) |