| RRC ID |
22708
|
| 著者 |
Gunaratne J, Schmidt A, Quandt A, Neo SP, Saraç OS, Gracia T, Loguercio S, Ahrné E, Xia RL, Tan KH, Lössner C, Bähler J, Beyer A, Blackstock W, Aebersold R.
|
| タイトル |
Extensive mass spectrometry-based analysis of the fission yeast proteome: the Schizosaccharomyces pombe PeptideAtlas.
|
| ジャーナル |
Mol Cell Proteomics
|
| Abstract |
We report a high quality and system-wide proteome catalogue covering 71% (3,542 proteins) of the predicted genes of fission yeast, Schizosaccharomyces pombe, presenting the largest protein dataset to date for this important model organism. We obtained this high proteome and peptide (11.4 peptides/protein) coverage by a combination of extensive sample fractionation, high resolution Orbitrap mass spectrometry, and combined database searching using the iProphet software as part of the Trans-Proteomics Pipeline. All raw and processed data are made accessible in the S. pombe PeptideAtlas. The identified proteins showed no biases in functional properties and allowed global estimation of protein abundances. The high coverage of the PeptideAtlas allowed correlation with transcriptomic data in a system-wide manner indicating that post-transcriptional processes control the levels of at least half of all identified proteins. Interestingly, the correlation was not equally tight for all functional categories ranging from r(s) >0.80 for proteins involved in translation to r(s) <0.45 for signal transduction proteins. Moreover, many proteins involved in DNA damage repair could not be detected in the PeptideAtlas despite their high mRNA levels, strengthening the translation-on-demand hypothesis for members of this protein class. In summary, the extensive and publicly available S. pombe PeptideAtlas together with the generated proteotypic peptide spectral library will be a useful resource for future targeted, in-depth, and quantitative proteomic studies on this microorganism.
|
| 巻・号 |
12(6)
|
| ページ |
1741-51
|
| 公開日 |
2013-6-1
|
| DOI |
10.1074/mcp.M112.023754
|
| PII |
S1535-9476(20)31089-6
|
| PMID |
23462206
|
| PMC |
PMC3675828
|
| MeSH |
Databases, Protein
Gene Expression Regulation, Fungal*
Mass Spectrometry
Multigene Family
Peptide Mapping
Peptides / isolation & purification*
Protein Processing, Post-Translational*
Proteome / chemistry
Proteome / genetics
Proteome / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism*
Schizosaccharomyces / chemistry
Schizosaccharomyces / genetics
Schizosaccharomyces / metabolism*
Schizosaccharomyces pombe Proteins / chemistry
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism*
Signal Transduction
|
| IF |
4.87
|
| 引用数 |
17
|
|
WOS 分野
|
BIOCHEMICAL RESEARCH METHODS
|
| リソース情報 |
| 酵母 |
FY7056 |
