RRC ID 22709
Author Mishra M, Kashiwazaki J, Takagi T, Srinivasan R, Huang Y, Balasubramanian MK, Mabuchi I.
Title In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics.
Journal Nat Cell Biol
Abstract Cytokinesis in many eukaryotes involves the contraction of an actomyosin-based contractile ring. However, the detailed mechanism of contractile ring contraction is not fully understood. Here, we establish an experimental system to study contraction of the ring to completion in vitro. We show that the contractile ring of permeabilized fission yeast cells undergoes rapid contraction in an ATP- and myosin-II-dependent manner in the absence of other cytoplasmic constituents. Surprisingly, neither actin polymerization nor its disassembly is required for contraction of the contractile ring, although addition of exogenous actin-crosslinking proteins blocks ring contraction. Using contractile rings generated from fission yeast cytokinesis mutants, we show that not all proteins required for assembly of the ring are required for its contraction in vitro. Our work provides the beginnings of the definition of a minimal contraction-competent cytokinetic ring apparatus.
Volume 15(7)
Pages 853-9
Published 2013-7
DOI 10.1038/ncb2781
PII ncb2781
PMID 23770677
MeSH Actins / metabolism* Adenosine Triphosphate / metabolism Cell Division Cell Membrane / metabolism Cytokinesis / physiology* Green Fluorescent Proteins / metabolism Myosin Type II / metabolism* Schizosaccharomyces / cytology* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / metabolism*
IF 17.728
Times Cited 57
Yeast FY14500 FY14718 FY14768 FY14771 FY14781 FY14793 FY14812 FY14933 FY14947 FY14957 FY14977 FY15014 FY15055 FY15061 FY15065 FY15226 FY15403 FY15505 FY15507 FY15526 FY17671(MT663) FY20237(MY6294) FY13991(YM012) FY21484(YO3)