Reference - Detail
RRC ID | 22721 |
---|---|
Author | Buchon N, Poidevin M, Kwon HM, Guillou A, Sottas V, Lee BL, Lemaitre B. |
Title | A single modular serine protease integrates signals from pattern-recognition receptors upstream of the Drosophila Toll pathway. |
Journal | Proc Natl Acad Sci U S A |
Abstract |
The Drosophila Toll receptor does not interact directly with microbial determinants, but is instead activated by a cleaved form of the cytokine-like molecule Spätzle. During the immune response, Spätzle is processed by complex cascades of serine proteases, which are activated by secreted pattern-recognition receptors. Here, we demonstrate the essential role of ModSP, a modular serine protease, in the activation of the Toll pathway by gram-positive bacteria and fungi. Our analysis shows that ModSP integrates signals originating from the circulating recognition molecules GNBP3 and PGRP-SA and connects them to the Grass-SPE-Spätzle extracellular pathway upstream of the Toll receptor. It also reveals the conserved role of modular serine proteases in the activation of insect immune reactions. |
Volume | 106(30) |
Pages | 12442-7 |
Published | 2009-7-28 |
DOI | 10.1073/pnas.0901924106 |
PII | 0901924106 |
PMID | 19590012 |
PMC | PMC2718337 |
MeSH | Amino Acid Sequence Animals Carrier Proteins / genetics Carrier Proteins / metabolism Cell Line Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Drosophila melanogaster / microbiology Fungi / physiology Gene Expression Gram-Positive Bacteria / physiology Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Host-Pathogen Interactions Intracellular Signaling Peptides and Proteins Molecular Sequence Data Mutation Receptors, Pattern Recognition / genetics Receptors, Pattern Recognition / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Serine Endopeptidases / genetics Serine Endopeptidases / metabolism* Signal Transduction* Spodoptera Toll-Like Receptors / genetics Toll-Like Receptors / metabolism* |
IF | 9.412 |
Times Cited | 110 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Drosophila |