| RRC ID |
22730
|
| 著者 |
Wu Y, Brock AR, Wang Y, Fujitani K, Ueda R, Galko MJ.
|
| タイトル |
A blood-borne PDGF/VEGF-like ligand initiates wound-induced epidermal cell migration in Drosophila larvae.
|
| ジャーナル |
Curr Biol
|
| Abstract |
Epidermal cell migration is critical for restoration of tissue structure and function after damage. However, the mechanisms by which differentiated cells neighboring the wound sense the wound and assume a motile phenotype remain unclear. Here, we show that Pvr, a receptor tyrosine kinase (RTK) related to platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) receptors, and one of its ligands, Pvf1, are required for epidermal wound closure. Morphological comparison of wound-edge cells lacking Pvr or the Jun N-terminal kinase (JNK) signaling pathway previously implicated in larval wound closure suggests that Pvr signaling leads wound-margin epidermal cells to extend actin-based cell processes into the wound gap while JNK mediates transient dedifferentiation of cells at the wound margin. Genetic epistasis experiments reinforce the conclusion that the JNK and Pvr signaling pathways act in parallel. Tissue-specific knockdown and rescue experiments suggest that epidermally derived Pvf1 may be sequestered in the blood and that tissue damage exposes blood-borne Pvf1 to Pvr receptors on wound-edge epidermal cells and initiates the extension of cell processes into the wound gap. These results uncover a novel mechanism of sensing tissue damage and suggest that PDGF/VEGF ligands and receptors may play a conserved autocrine role in epidermal wound closure.
|
| 巻・号 |
19(17)
|
| ページ |
1473-7
|
| 公開日 |
2009-9-15
|
| DOI |
10.1016/j.cub.2009.07.019
|
| PII |
S0960-9822(09)01401-8
|
| PMID |
19646875
|
| PMC |
PMC2783944
|
| MeSH |
Animals
Cell Movement / physiology*
Drosophila / cytology
Drosophila / embryology*
Drosophila / physiology
Drosophila Proteins / genetics
Drosophila Proteins / metabolism
Drosophila Proteins / physiology*
Egg Proteins / genetics
Egg Proteins / metabolism
Egg Proteins / physiology*
Epistasis, Genetic
Hemolymph / metabolism
JNK Mitogen-Activated Protein Kinases
Larva / cytology
Larva / physiology
Ligands
MAP Kinase Signaling System
Models, Biological
RNA Interference
Receptor Protein-Tyrosine Kinases / genetics
Receptor Protein-Tyrosine Kinases / metabolism
Receptor Protein-Tyrosine Kinases / physiology*
|
| IF |
9.601
|
| 引用数 |
47
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
5680R-1
5680R-2
13780R-1
13780R-2
13781R-1
8222R-3 |
