論文 - 詳細
| RRC ID | 22737 |
|---|---|
| 著者 | Wang L, Kiuchi T, Fujii T, Daimon T, Li M, Banno Y, Kikuta S, Kikawada T, Katsuma S, Shimada T. |
| タイトル | Mutation of a novel ABC transporter gene is responsible for the failure to incorporate uric acid in the epidermis of ok mutants of the silkworm, Bombyx mori. |
| ジャーナル | Insect Biochem Mol Biol |
| Abstract |
ok mutants of the silkworm, Bombyx mori, exhibit highly translucent larval skin resulting from the inability to incorporate uric acid into the epidermal cells. Here we report the identification of a gene responsible for the ok mutation using positional cloning and RNAi experiments. In two independent ok mutant strains, we found a 49-bp deletion and a 233-bp duplication, respectively, in mRNAs of a novel gene, Bm-ok, which encodes a half-type ABC transporter, each of which results in translation of a truncated protein in each mutant. Although the Bm-ok sequence was homologous to well-known transporter genes, white, scarlet, and brown in Drosophila, the discovery of novel orthologs in the genomes of lepidopteran, hymenopteran, and hemipteran insects identifies it as a member of a new distinct subfamily of transporters. Embryonic RNAi of Bm-ok demonstrated that repression of Bm-ok causes a translucent phenotype in the first-instar silkworm larva. We discuss the possibility that Bm-ok forms a heterodimer with another half-type ABC transporter, Bmwh3, and acts as a uric acid transporter in the silkworm epidermis. |
| 巻・号 | 43(7) |
| ページ | 562-71 |
| 公開日 | 2013-7-1 |
| DOI | 10.1016/j.ibmb.2013.03.011 |
| PII | S0965-1748(13)00054-4 |
| 解説 | ok(金鶏竜油)の原因遺伝子はBm-okである。Bm-okはABCトランスポーターfamilyに属するタンパク質をコードする。o21系統では、Bm-okのエクソン３が欠失している。o22系統では、エクソン３と４が重複している。 |
| PMID | 23567590 |
| MeSH | ATP-Binding Cassette Transporters / genetics* ATP-Binding Cassette Transporters / metabolism* Animals Biological Transport Bombyx / classification Bombyx / enzymology* Bombyx / genetics Bombyx / metabolism Cloning, Molecular Epidermis / enzymology Epidermis / metabolism Female Insect Proteins / genetics* Insect Proteins / metabolism* Insecta / classification Insecta / enzymology Insecta / genetics Male Mutation Phylogeny Uric Acid / metabolism* |
| IF | 3.827 |
| 引用数 | 30 |
| WOS 分野 | ENTOMOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カイコ | o21 o22 p50 |