RRC ID 22739
著者 Banno Y, Nagasaki K, Tsukada M, Minohara Y, Banno J, Nishikawa K, Yamamoto K, Tamura K, Fujii T.
タイトル Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries.
ジャーナル Cryobiology
Abstract Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1 °C per min) until -80 °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7 ± 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3 ± 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4 ± 21.6% vs 22.9 ± 9.3%).
巻・号 66(3)
ページ 283-7
公開日 2013-6-1
DOI 10.1016/j.cryobiol.2013.03.004
PII S0011-2240(13)00111-9
PMID 23500076
MeSH Animals Bombyx / genetics Bombyx / physiology* Cryopreservation / methods Cryopreservation / veterinary* Female Freezing Larva / physiology Ovary / physiology* Ovary / transplantation* Zygote / physiology
IF 2.287
引用数 6
WOS 分野 BIOLOGY PHYSIOLOGY
リソース情報
カイコ xe28 r03 p20 p50