| RRC ID |
22741
|
| Author |
Tatsuke T, Lee JM, Kusakabe T, Iiyama K, Sezutsu H, Uchino K.
|
| Title |
Tightly controlled tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells.
|
| Journal |
Arch Insect Biochem Physiol
|
| Abstract |
Tetracycline-inducible gene expression (Tet-on) system is a particularly powerful tool for transgenic research and has become one of the first choices for the control of transgene expression in a mammal and a fly. Previously, we have generated the modified reverse tetracycline-controlled transactivators and tetO promoters for a Bombyx mori Tet-on system. In order to further improve this system, Giant, a transcriptional silencer from Drosophila melanogaster, is introduced to repress leaky transcription in the absence of doxycycline. Further, the promoter responsibility to the tetracycline-controlled transactivators is facilitated by introducing a synthetic minimal core promoter. With the tightly regulated second-generation silkworm Tet-on system, we obtain up to 300-fold induction of gene expression with the addition of doxycycline.
|
| Volume |
82(4)
|
| Pages |
173-82
|
| Published |
2013-4-1
|
| DOI |
10.1002/arch.21083
|
| PMID |
23371880
|
| MeSH |
Animals
Bombyx / cytology
Bombyx / metabolism*
Cells, Cultured
Gene Expression Regulation
Genes, Reporter
Genetic Techniques*
Nuclear Localization Signals
Promoter Regions, Genetic
RNA Interference
Tetracycline
Trans-Activators*
Transcription, Genetic*
|
| IF |
1.536
|
| Times Cited |
3
|
|
WOS Category
|
ENTOMOLOGY
PHYSIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Silkworms |
|
