論文 - 詳細
| RRC ID | 22742 |
|---|---|
| 著者 | Osanai-Futahashi M, Ohde T, Hirata J, Uchino K, Futahashi R, Tamura T, Niimi T, Sezutsu H. |
| タイトル | A visible dominant marker for insect transgenesis. |
| ジャーナル | Nat Commun |
| Abstract |
Transgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa. |
| 巻・号 | 3 |
| ページ | 1295 |
| 公開日 | 2012-1-1 |
| DOI | 10.1038/ncomms2312 |
| PII | ncomms2312 |
| PMID | 23250425 |
| PMC | PMC3535423 |
| MeSH | Animals Animals, Genetically Modified / anatomy & histology Animals, Genetically Modified / genetics Arthropod Antennae / anatomy & histology Arylalkylamine N-Acetyltransferase / genetics Arylalkylamine N-Acetyltransferase / metabolism Bombyx / anatomy & histology Bombyx / genetics* Drosophila melanogaster / anatomy & histology Drosophila melanogaster / genetics Gene Transfer Techniques* Genetic Markers / genetics Insect Proteins / genetics Insecta / anatomy & histology Insecta / genetics Larva / anatomy & histology Larva / genetics Life Cycle Stages / genetics Phenotype Pigmentation / genetics |
| IF | 12.121 |
| 引用数 | 27 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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