RRC ID 22746
著者 Atsumi S, Miyamoto K, Yamamoto K, Narukawa J, Kawai S, Sezutsu H, Kobayashi I, Uchino K, Tamura T, Mita K, Kadono-Okuda K, Wada S, Kanda K, Goldsmith MR, Noda H.
タイトル Single amino acid mutation in an ATP-binding cassette transporter gene causes resistance to Bt toxin Cry1Ab in the silkworm, Bombyx mori.
ジャーナル Proc Natl Acad Sci U S A
Abstract Bt toxins derived from the arthropod bacterial pathogen Bacillus thuringiensis are widely used for insect control as insecticides or in transgenic crops. Bt resistance has been found in field populations of several lepidopteran pests and in laboratory strains selected with Bt toxin. Widespread planting of crops expressing Bt toxins has raised concerns about the potential increase of resistance mutations in targeted insects. By using Bombyx mori as a model, we identified a candidate gene for a recessive form of resistance to Cry1Ab toxin on chromosome 15 by positional cloning. BGIBMGA007792-93, which encodes an ATP-binding cassette transporter similar to human multidrug resistance protein 4 and orthologous to genes associated with recessive resistance to Cry1Ac in Heliothis virescens and two other lepidopteran species, was expressed in the midgut. Sequences of 10 susceptible and seven resistant silkworm strains revealed a common tyrosine insertion in an outer loop of the predicted transmembrane structure of resistant alleles. We confirmed the role of this ATP-binding cassette transporter gene in Bt resistance by converting a resistant silkworm strain into a susceptible one by using germline transformation. This study represents a direct demonstration of Bt resistance gene function in insects with the use of transgenesis.
巻・号 109(25)
ページ E1591-8
公開日 2012-6-19
DOI 10.1073/pnas.1120698109
PII 1120698109
PMID 22635270
PMC PMC3382473
MeSH ATP-Binding Cassette Transporters / chemistry ATP-Binding Cassette Transporters / genetics* Amino Acid Sequence Amino Acid Substitution* Animals Bacillus thuringiensis Toxins Bacterial Proteins / pharmacology* Bombyx / genetics* Chromosome Mapping Endotoxins / pharmacology* Genetic Linkage Hemolysin Proteins / pharmacology* Insecticide Resistance / genetics* Molecular Sequence Data Mutation* Polymorphism, Single Nucleotide Sequence Homology, Amino Acid
IF 9.412
引用数 127
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
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