RRC ID 22763
著者 Yamaguchi J, Mizoguchi T, Fujiwara H.
タイトル siRNAs induce efficient RNAi response in Bombyx mori embryos.
ジャーナル PLoS One
Abstract Short interference RNA (siRNA) is widely used in mammalian cells. In insects, however, reports concerning the suitablility of siRNA in vivo is very limited compared with that of long dsRNA, which is thought to be more effective. There is insufficient information on the essential rules of siRNA design in insects, as very few siRNAs have been tested in this context. To establish an effective method of gene silencing using siRNA in vivo in insects, we determined the effects of siRNA on seven target genes. We designed siRNAs according to a new guideline and injected them into eggs of Bombyx mori. At the mRNA level, the expression of most of these genes was successfully silenced, down to less than half the constitutive level, which in some cases led to the development of distinctive phenotypes. In addition, we observed stronger effect of siRNA both on the mRNA level and the phenotype than that of long dsRNA under comparable conditions. These results indicate that direct injection of siRNA is an effective reverse-genetics tool for the analysis of embryogenesis in vivo in insects.
巻・号 6(9)
ページ e25469
公開日 2011-1-1
DOI 10.1371/journal.pone.0025469
PII PONE-D-11-08358
PMID 21980469
PMC PMC3184131
MeSH Animals Base Composition Bombyx / embryology* Bombyx / genetics* Embryo, Nonmammalian / metabolism* Genes, Insect / genetics Injections Ovum / metabolism Phenotype RNA Interference* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / administration & dosage RNA, Small Interfering / genetics* Time Factors
IF 2.74
引用数 40
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
カイコ