RRC ID 22778
著者 Li Z, Zhu L, Xu J, Mon H, Lee JM, Kusakabe T.
タイトル Amino Acid deprivation-induced expression of asparagine synthetase regulates the growth and survival of cultured silkworm cells.
ジャーナル Arch Insect Biochem Physiol
Abstract Expression of Bombyx mori Asparagine synthetase (BmASNS), one gene that encodes an enzyme catalyzing asparagine biosynthesis, is transcriptionally induced following amino acid deprivation. Previous transcriptional analysis of the BmASNS gene showed the involvement of Polycomb proteins, epigenetic repressors, in suppressing BmASNS expression in a cell cycle-dependent manner. However, the role of BmAsns protein in these cellular processes remains unclear. The present study thus exploited the potential function of BmAsns protein in cultured silkworm cells. Our results showed that ectopic overexpression of BmASNS gene effectively inhibited cell growth in silkworm cells, whereas its overexpression could rescue cell growth upon amino acid deprivation treatment. We found that the cells expressing BmAsns protein were capable of influencing the formation of autophagic vacuoles stimulated by amino acid deprivation. We speculated that the recovery of cell growth by overexpressed BmAsns protein is due to the rapid turnover of autophagic vacuoles in the cells. To further assess the effects of BmAsns on cell development, we used RNA interference to silence BmASNS expression in silkworm cells in the presence or absence of amino acids. Our results revealed a significant change of cell proliferation as well as cell cycle distribution after knockdown of BmASNS. Importantly, silkworm cells lacking BmASNS under the condition of amino acid deprivation showed severely impaired proliferation. Altogether, we concluded that the up-regulated expression of BmASNS would be able to protect cells from impairment induced by amino acid deprivation, which in turn facilitates cell growth and survival.
巻・号 83(2)
ページ 57-68
公開日 2013-6-1
DOI 10.1002/arch.21091
PMID 23633098
MeSH Amino Acids / metabolism* Animals Aspartate-Ammonia Ligase / genetics Aspartate-Ammonia Ligase / metabolism* Bombyx / cytology* Bombyx / enzymology Bombyx / genetics Bombyx / physiology Cells, Cultured Gene Expression Regulation Gene Knockdown Techniques Luminescent Proteins / metabolism Microscopy, Fluorescence RNA Interference Reverse Transcriptase Polymerase Chain Reaction
IF 1.536
引用数 1
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY ENTOMOLOGY PHYSIOLOGY
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