RRC ID |
22778
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著者 |
Li Z, Zhu L, Xu J, Mon H, Lee JM, Kusakabe T.
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タイトル |
Amino Acid deprivation-induced expression of asparagine synthetase regulates the growth and survival of cultured silkworm cells.
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ジャーナル |
Arch Insect Biochem Physiol
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Abstract |
Expression of Bombyx mori Asparagine synthetase (BmASNS), one gene that encodes an enzyme catalyzing asparagine biosynthesis, is transcriptionally induced following amino acid deprivation. Previous transcriptional analysis of the BmASNS gene showed the involvement of Polycomb proteins, epigenetic repressors, in suppressing BmASNS expression in a cell cycle-dependent manner. However, the role of BmAsns protein in these cellular processes remains unclear. The present study thus exploited the potential function of BmAsns protein in cultured silkworm cells. Our results showed that ectopic overexpression of BmASNS gene effectively inhibited cell growth in silkworm cells, whereas its overexpression could rescue cell growth upon amino acid deprivation treatment. We found that the cells expressing BmAsns protein were capable of influencing the formation of autophagic vacuoles stimulated by amino acid deprivation. We speculated that the recovery of cell growth by overexpressed BmAsns protein is due to the rapid turnover of autophagic vacuoles in the cells. To further assess the effects of BmAsns on cell development, we used RNA interference to silence BmASNS expression in silkworm cells in the presence or absence of amino acids. Our results revealed a significant change of cell proliferation as well as cell cycle distribution after knockdown of BmASNS. Importantly, silkworm cells lacking BmASNS under the condition of amino acid deprivation showed severely impaired proliferation. Altogether, we concluded that the up-regulated expression of BmASNS would be able to protect cells from impairment induced by amino acid deprivation, which in turn facilitates cell growth and survival.
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巻・号 |
83(2)
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ページ |
57-68
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公開日 |
2013-6-1
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DOI |
10.1002/arch.21091
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PMID |
23633098
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MeSH |
Amino Acids / metabolism*
Animals
Aspartate-Ammonia Ligase / genetics
Aspartate-Ammonia Ligase / metabolism*
Bombyx / cytology*
Bombyx / enzymology
Bombyx / genetics
Bombyx / physiology
Cells, Cultured
Gene Expression Regulation
Gene Knockdown Techniques
Luminescent Proteins / metabolism
Microscopy, Fluorescence
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
|
IF |
1.536
|
引用数 |
1
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WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
ENTOMOLOGY
PHYSIOLOGY
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リソース情報 |
カイコ |
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