| RRC ID |
22782
|
| 著者 |
Li Z, Cheng D, Mon H, Zhu L, Xu J, Tatsuke T, Lee JM, Xia Q, Kusakabe T.
|
| タイトル |
Cell cycle-dependent recruitment of polycomb proteins to the ASNS promoter counteracts C/ebp-mediated transcriptional activation in Bombyx mori.
|
| ジャーナル |
PLoS One
|
| Abstract |
Epigenetic modifiers and transcription factors contribute to developmentally programmed gene expression. Here, we establish a functional link between epigenetic regulation by Polycomb group (PcG) proteins and transcriptional regulation by C/ebp that orchestrates the correct expression of Bombyx mori asparagine synthetase (BmASNS), a gene involved in the biosynthesis of asparagine. We show that the cis-regulatory elements of YY1-binding motifs and the CpG island present on the BmASNS promoter are required for the recruitment of PcG proteins and the subsequent deposition of the epigenetic repression mark H3K27me3. RNAi-mediated knockdown of PcG genes leads to derepression of the BmASNS gene via the recruitment of activators, including BmC/ebp, to the promoter. Intriguingly, we find that PcG proteins and BmC/ebp can dynamically modulate the transcriptional output of the BmASNS target in a cell cycle-dependent manner. It will be essential to suppress BmASNS expression by PcG proteins at the G2/M phase of the cell cycle in the presence of BmC/ebp activator. Thus, our results provide a novel insight into the molecular mechanism underlying the recruitment and regulation of the PcG system at a discrete gene locus in Bombyx mori.
|
| 巻・号 |
8(1)
|
| ページ |
e52320
|
| 公開日 |
2013-1-1
|
| DOI |
10.1371/journal.pone.0052320
|
| PII |
PONE-D-12-24668
|
| PMID |
23382816
|
| PMC |
PMC3557315
|
| MeSH |
Animals
Asparagine / biosynthesis
Aspartate-Ammonia Ligase / genetics*
Aspartate-Ammonia Ligase / metabolism
Bombyx / enzymology*
CCAAT-Enhancer-Binding Proteins / genetics*
CCAAT-Enhancer-Binding Proteins / metabolism
Cell Cycle Checkpoints / genetics
CpG Islands / genetics
Epigenesis, Genetic
Gene Expression Regulation, Developmental
Polycomb-Group Proteins* / genetics
Polycomb-Group Proteins* / metabolism
Promoter Regions, Genetic
Transcriptional Activation / genetics
|
| IF |
2.74
|
| 引用数 |
8
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
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| リソース情報 |
| カイコ |
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