論文 - 詳細
| RRC ID | 22782 |
|---|---|
| 著者 | Li Z, Cheng D, Mon H, Zhu L, Xu J, Tatsuke T, Lee JM, Xia Q, Kusakabe T. |
| タイトル | Cell cycle-dependent recruitment of polycomb proteins to the ASNS promoter counteracts C/ebp-mediated transcriptional activation in Bombyx mori. |
| ジャーナル | PLoS One |
| Abstract |
Epigenetic modifiers and transcription factors contribute to developmentally programmed gene expression. Here, we establish a functional link between epigenetic regulation by Polycomb group (PcG) proteins and transcriptional regulation by C/ebp that orchestrates the correct expression of Bombyx mori asparagine synthetase (BmASNS), a gene involved in the biosynthesis of asparagine. We show that the cis-regulatory elements of YY1-binding motifs and the CpG island present on the BmASNS promoter are required for the recruitment of PcG proteins and the subsequent deposition of the epigenetic repression mark H3K27me3. RNAi-mediated knockdown of PcG genes leads to derepression of the BmASNS gene via the recruitment of activators, including BmC/ebp, to the promoter. Intriguingly, we find that PcG proteins and BmC/ebp can dynamically modulate the transcriptional output of the BmASNS target in a cell cycle-dependent manner. It will be essential to suppress BmASNS expression by PcG proteins at the G2/M phase of the cell cycle in the presence of BmC/ebp activator. Thus, our results provide a novel insight into the molecular mechanism underlying the recruitment and regulation of the PcG system at a discrete gene locus in Bombyx mori. |
| 巻・号 | 8(1) |
| ページ | e52320 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0052320 |
| PII | PONE-D-12-24668 |
| PMID | 23382816 |
| PMC | PMC3557315 |
| MeSH | Animals Asparagine / biosynthesis Aspartate-Ammonia Ligase / genetics* Aspartate-Ammonia Ligase / metabolism Bombyx / enzymology* CCAAT-Enhancer-Binding Proteins / genetics* CCAAT-Enhancer-Binding Proteins / metabolism Cell Cycle Checkpoints / genetics CpG Islands / genetics Epigenesis, Genetic Gene Expression Regulation, Developmental Polycomb-Group Proteins* / genetics Polycomb-Group Proteins* / metabolism Promoter Regions, Genetic Transcriptional Activation / genetics |
| IF | 2.74 |
| 引用数 | 8 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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