論文 - 詳細
| RRC ID | 22796 |
|---|---|
| 著者 | Fujiyuki T, Hamamoto H, Ishii K, Urai M, Kataoka K, Takeda T, Shibata S, Sekimizu K. |
| タイトル | Evaluation of innate immune stimulating activity of polysaccharides using a silkworm (Bombyx mori) muscle contraction assay. |
| ジャーナル | Drug Discov Ther |
| Abstract |
In silkworm larvae, the mature form of paralytic peptide (PP), an insect cytokine, is produced from pro-PP in association with activation of innate immune responses, resulting in slow muscle contraction. We utilized this reaction, muscle contraction in silkworms coupled with innate immunity stimulation, to quantitatively measure the innate immune stimulating activity of various natural polysaccharides. β-Glucan of Gyrophora esculenta (GE-3), fucoidan from sporophyll of Undaria pinnatifida, and curldan induced silkworm muscle contraction. We further demonstrated that GE-3 had therapeutic effects on silkworms infected by baculovirus. Based on these findings, we propose that the silkworm muscle contraction assay is useful for screening substances that stimulate innate immunity before evaluating therapeutic effectiveness in mammals. |
| 巻・号 | 6(2) |
| ページ | 88-93 |
| 公開日 | 2012-4-1 |
| PII | 540 |
| PMID | 22622018 |
| MeSH | Animals Bombyx Drug Evaluation, Preclinical / methods* Hemolymph / metabolism Immunity, Innate / drug effects* Larva / drug effects Muscle Contraction / drug effects* Neuropeptides / metabolism Nucleopolyhedroviruses / drug effects Polysaccharides / immunology* Polysaccharides / pharmacology* beta-Glucans / pharmacology |
| 引用数 | 11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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