RRC ID 22812
著者 Usui K, Miyazaki S, Kaito C, Sekimizu K.
タイトル Purification of a soil bacteria exotoxin using silkworm toxicity to measure specific activity.
ジャーナル Microb Pathog
Abstract Overnight cultures of 16 of 25 species of soil bacterium injected into silkworm hemolymph killed the silkworms. Injection of culture supernatants of five of these 16 bacterial species into the hemolymph also killed silkworms. Analysis of 16S rRNA indicated that the toxic species were Bacillus species. We purified an exotoxin produced by one of the Bacillus species by measuring the specific activity based on silkworm toxicity. The final step of purification revealed that the silkworm-killing activity was due to a 34-kDa protein. LC/MS/MS analysis revealed that the 34-kDa protein is sphingomyelinase C of Bacillus cereus. We propose that the silkworm infection model is useful for identifying pathogenic bacteria from soil and purifying bacterial toxins.
巻・号 46(2)
ページ 59-62
公開日 2009-2-1
DOI 10.1016/j.micpath.2008.10.010
PII S0882-4010(08)00137-X
PMID 19041713
MeSH Animals Bacillus / classification Bacillus / genetics Bacillus / isolation & purification Bacillus / pathogenicity* Bacterial Toxins* / chemistry Bacterial Toxins* / isolation & purification Bacterial Toxins* / metabolism Bacterial Toxins* / toxicity Bacterial Typing Techniques Bombyx / microbiology* DNA, Bacterial / analysis Exotoxins* / chemistry Exotoxins* / isolation & purification Exotoxins* / metabolism Exotoxins* / toxicity Hemolymph / microbiology RNA, Ribosomal, 16S / genetics Soil Microbiology* Sphingomyelin Phosphodiesterase / metabolism
IF 2.914
引用数 15
WOS 分野 MICROBIOLOGY IMMUNOLOGY
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