論文 - 詳細
| RRC ID | 22831 |
|---|---|
| 著者 | Signor S, Seher T, Kopp A. |
| タイトル | Genomic resources for multiple species in the Drosophila ananassae species group. |
| ジャーナル | Fly (Austin) |
| Abstract |
The development of genomic resources in non-model taxa is essential for understanding the genetic basis of biological diversity. Although the genomes of many Drosophila species have been sequenced, most of the phenotypic diversity in this genus remains to be explored. To facilitate the genetic analysis of interspecific and intraspecific variation, we have generated new genomic resources for seven species and subspecies in the D. ananassae species subgroup. We have generated large amounts of transcriptome sequence data for D. ercepeae, D. merina, D. bipectinata, D. malerkotliana malerkotliana, D. m. pallens, D. pseudoananassae pseudoananassae, and D. p. nigrens. de novo assembly resulted in contigs covering more than half of the predicted transcriptome and matching an average of 59% of annotated genes in the complete genome of D. ananassae. Most contigs, corresponding to an average of 49% of D. ananassae genes, contain sequence polymorphisms that can be used as genetic markers. Subsets of these markers were validated by genotyping the progeny of inter- and intraspecific crosses. The ananassae subgroup is an excellent model system for examining the molecular basis of speciation and phenotypic evolution. The new genomic resources will facilitate the genetic analysis of inter- and intraspecific differences in this lineage. Transcriptome sequencing provides a simple and cost-effective way to identify molecular markers at nearly single-gene density, and is equally applicable to any non-model taxa. |
| 巻・号 | 7(1) |
| ページ | 47-57 |
| 公開日 | 2013-1-1 |
| DOI | 10.4161/fly.22353 |
| PII | 22353 |
| PMID | 23639891 |
| PMC | PMC3660750 |
| MeSH | Animals Chromosome Mapping Chromosomes, Insect Crosses, Genetic Drosophila / genetics* Gene Library* Genetic Markers Genome* Hybridization, Genetic Polymorphism, Single Nucleotide Species Specificity Transcriptome* |
| IF | 1.204 |
| 引用数 | 6 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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