RRC ID 2284
Author Nakade K, Pan J, Yoshiki A, Ugai H, Kimura M, Liu B, Li H, Obata Y, Iwama M, Itohara S, Murata T, Yokoyama KK.
Title JDP2 suppresses adipocyte differentiation by regulating histone acetylation.
Journal Cell Death Differ
Abstract Among the events that control cellular differentiation, the acetylation of histones plays a critical role in the regulation of transcription and the modification of chromatin. Jun dimerization protein 2 (JDP2), a member of the AP-1 family, is an inhibitor of such acetylation and contributes to the maintenance of chromatin structure. In an examination of Jdp2 'knock-out' (KO) mice, we observed elevated numbers of white adipocytes and significant accumulation of lipid in the adipose tissue in sections of scapulae. In addition, mouse embryo fibroblasts (MEFs) from Jdp2 KO mice were more susceptible to adipocyte differentiation in response to hormonal induction and members of the CCAAT/enhancer-binding proteins (C/EBP) gene family were expressed at levels higher than MEFs from wild-type mice. Furthermore, JDP2 inhibited both the acetylation of histone H3 in the promoter of the gene for C/EBPdelta and transcription from this promoter. Our data indicate that JDP2 plays a key role as a repressor of adipocyte differentiation by regulating the expression of the gene for C/EBPdelta via inhibition of histone acetylation.
Volume 14(8)
Pages 1398-405
Published 2007-8-1
DOI 10.1038/sj.cdd.4402129
PII 4402129
PMID 17464331
MeSH 3T3-L1 Cells Acetylation Adipocytes / cytology* Adipocytes / metabolism* Adipogenesis / genetics Adipogenesis / physiology Animals Base Sequence CCAAT-Enhancer-Binding Protein-delta / genetics Cell Differentiation / physiology DNA Primers / genetics Female Fibroblasts / cytology Fibroblasts / metabolism Gene Targeting Histones / chemistry Histones / metabolism* In Vitro Techniques Male Mice Mice, Inbred C57BL Mice, Knockout Mutation Pregnancy Promoter Regions, Genetic Repressor Proteins / genetics Repressor Proteins / physiology*
IF 10.717
Times Cited 32
DNA material AxCALacZ (RDB01745)
Mice RBRC00861