論文 - 詳細
| RRC ID | 2359 |
|---|---|
| 著者 | Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y. |
| タイトル | Refinement of cytokine use in the in vitro expansion of erythroid cells. |
| ジャーナル | Hum Cell |
| Abstract |
Blood transfusion is indispensable for many clinical applications. However, the supply of transfusable material is insufficient in many countries. Human cord blood contains many hematopoietic stem and progenitor cells, providing a promising resource for the production of transfusable material in vitro. In this study, we have refined a protocol to produce abundant red blood cells (RBC) from human cord blood in an in vitro culture system. We found that erythropoietin and interleukin-3 were most effective when they were added to the culture medium sequentially rather than simultaneously. Although insulin-like growth factor-I (IGF-1) has been reported to function as a positive regulator of RBC production in some in vitro culture systems, we found that IGF-1 had a negative effect upon RBC production. However, IGF-II appeared to function as a positive regulator of RBC production. Finally, stem cell factor functioned to both expand and accelerate the differentiation of immature erythroid cells. |
| 巻・号 | 19(1) |
| ページ | 30-7 |
| 公開日 | 2006-2-1 |
| DOI | 10.1111/j.1749-0774.2005.00005.x |
| PII | HUC |
| PMID | 16643605 |
| MeSH | Animals Cell Culture Techniques / methods* Cell Differentiation / drug effects* Cells, Cultured Cytokines / administration & dosage Cytokines / pharmacology* Erythrocytes / cytology* Erythropoiesis / drug effects* Female Fetal Blood / cytology* Humans Mice Mice, Inbred C57BL Somatomedins / pharmacology Stimulation, Chemical |
| IF | 3.463 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 研究用ヒト臍帯血幹細胞 | |