| RRC ID |
2359
|
| 著者 |
Miharada K, Hiroyama T, Sudo K, Nagasawa T, Nakamura Y.
|
| タイトル |
Refinement of cytokine use in the in vitro expansion of erythroid cells.
|
| ジャーナル |
Hum Cell
|
| Abstract |
Blood transfusion is indispensable for many clinical applications. However, the supply of transfusable material is insufficient in many countries. Human cord blood contains many hematopoietic stem and progenitor cells, providing a promising resource for the production of transfusable material in vitro. In this study, we have refined a protocol to produce abundant red blood cells (RBC) from human cord blood in an in vitro culture system. We found that erythropoietin and interleukin-3 were most effective when they were added to the culture medium sequentially rather than simultaneously. Although insulin-like growth factor-I (IGF-1) has been reported to function as a positive regulator of RBC production in some in vitro culture systems, we found that IGF-1 had a negative effect upon RBC production. However, IGF-II appeared to function as a positive regulator of RBC production. Finally, stem cell factor functioned to both expand and accelerate the differentiation of immature erythroid cells.
|
| 巻・号 |
19(1)
|
| ページ |
30-7
|
| 公開日 |
2006-2-1
|
| DOI |
10.1111/j.1749-0774.2005.00005.x
|
| PII |
HUC
|
| PMID |
16643605
|
| MeSH |
Animals
Cell Culture Techniques / methods*
Cell Differentiation / drug effects*
Cells, Cultured
Cytokines / administration & dosage
Cytokines / pharmacology*
Erythrocytes / cytology*
Erythropoiesis / drug effects*
Female
Fetal Blood / cytology*
Humans
Mice
Mice, Inbred C57BL
Somatomedins / pharmacology
Stimulation, Chemical
|
| IF |
3.463
|
| リソース情報 |
| 研究用ヒト臍帯血幹細胞 |
|
