RRC ID 2365
著者 Maki J, Masuda C, Morikawa S, Morita M, Inubushi T, Matsusue Y, Taguchi H, Tooyama I.
タイトル The MR tracking of transplanted ATDC5 cells using fluorinated poly-L-lysine-CF3.
ジャーナル Biomaterials
Abstract Magnetic resonance (MR) imaging using super-paramagnetic iron oxides (SPIOs) is a powerful tool to monitor transplanted cells in living animals. However, since SPIOs are negative contrast agents it is difficult to track transplanted cells in bone and cartilage that originally display low signals. In this study, we examined the feasibility of tracking with fluorescein isothiocyanate (FITC)-labeled poly-L-lysine-CF(3) (PLK-CF(3)) using mouse ATDC5 cells, a stem cell line of bone and cartilage cells. FITC-labeled PLK-CF(3) was easily internalized by ATDC5 cells by adding it into culture medium. No acute or long-term toxicities were seen at less than 160 microg/ml. Labeled cells transplanted into the cranial bone of mice were detected for at least 7 days by MR images. FITC-labeled PLK-CF(3) is a useful positive contrast agent for MR tracking in bone and cartilage.
巻・号 28(3)
ページ 434-40
公開日 2007-1-1
DOI 10.1016/j.biomaterials.2006.08.050
PII S0142-9612(06)00762-9
PMID 16978693
MeSH Animals Bone Transplantation / methods* Cell Line Cell Transplantation / methods* Cells, Cultured Contrast Media / pharmacology Ferrosoferric Oxide / pharmacology* Hydrogen-Ion Concentration Magnetic Resonance Imaging / methods* Magnetic Resonance Spectroscopy Male Mice Mice, Inbred ICR Polylysine / pharmacology* Sensitivity and Specificity Skull / pathology*
IF 10.317
引用数 22
WOS 分野 ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS
リソース情報
ヒト・動物細胞