論文 - 詳細
| RRC ID | 2368 |
|---|---|
| 著者 | Ushiro Y, Hashimoto O, Seki M, Hachiya A, Shoji H, Hasegawa Y. |
| タイトル | Analysis of the function of activin betaC subunit using recombinant protein. |
| ジャーナル | J Reprod Dev |
| Abstract |
Activins, TGF-beta superfamily members, have multiple functions in a variety of cells and tissues. Additional activin beta subunit genes, betaC and betaE, have been identified in humans and rodents. To explore the role of activin betaC subunit, we generated recombinant human activin C using Chinese hamster ovary cells. Recombinant activin C from the conditioned medium was purified by consecutive hydrophobic, size-exclusion, and high performance liquid chromatography. SDS-PAGE and Western blot analysis of the purified protein revealed that activin C formed disulfide bridges. However, activin C had no effect on the proliferation of cultured liver cells. Furthermore, there were no significant differences in erythroid differentiation and follicle stimulating hormone secretion in vitro. It was also shown that immunoreactive bands indicated the hetrodimer of activin betaC, and inhibin alpha subunits were detected in the conditioned medium from the activin C-producing cells, which were stably transfected with inhibin alpha subunit cDNA. This suggests that activin betaC subunit may have been present and that it may exert its effect as inhibin C. |
| 巻・号 | 52(4) |
| ページ | 487-95 |
| 公開日 | 2006-8-1 |
| DOI | 10.1262/jrd.17110 |
| PII | JST.JSTAGE/jrd/17110 |
| PMID | 16627954 |
| MeSH | Animals CHO Cells Cell Differentiation Cricetinae Cricetulus Dimerization Dose-Response Relationship, Drug Follicle Stimulating Hormone / metabolism Genetic Techniques Humans Inhibin-beta Subunits / analysis* Inhibin-beta Subunits / physiology* Inhibins / chemistry K562 Cells Liver / metabolism Rats Recombinant Proteins / analysis* Recombinant Proteins / chemistry |
| IF | 1.735 |
| 引用数 | 11 |
| WOS 分野 | AGRICULTURE, DAIRY & ANIMAL SCIENCE REPRODUCTIVE BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |