Reference - Detail
| RRC ID | 2368 |
|---|---|
| Author | Ushiro Y, Hashimoto O, Seki M, Hachiya A, Shoji H, Hasegawa Y. |
| Title | Analysis of the function of activin betaC subunit using recombinant protein. |
| Journal | J Reprod Dev |
| Abstract |
Activins, TGF-beta superfamily members, have multiple functions in a variety of cells and tissues. Additional activin beta subunit genes, betaC and betaE, have been identified in humans and rodents. To explore the role of activin betaC subunit, we generated recombinant human activin C using Chinese hamster ovary cells. Recombinant activin C from the conditioned medium was purified by consecutive hydrophobic, size-exclusion, and high performance liquid chromatography. SDS-PAGE and Western blot analysis of the purified protein revealed that activin C formed disulfide bridges. However, activin C had no effect on the proliferation of cultured liver cells. Furthermore, there were no significant differences in erythroid differentiation and follicle stimulating hormone secretion in vitro. It was also shown that immunoreactive bands indicated the hetrodimer of activin betaC, and inhibin alpha subunits were detected in the conditioned medium from the activin C-producing cells, which were stably transfected with inhibin alpha subunit cDNA. This suggests that activin betaC subunit may have been present and that it may exert its effect as inhibin C. |
| Volume | 52(4) |
| Pages | 487-95 |
| Published | 2006-8-1 |
| DOI | 10.1262/jrd.17110 |
| PII | JST.JSTAGE/jrd/17110 |
| PMID | 16627954 |
| MeSH | Animals CHO Cells Cell Differentiation Cricetinae Cricetulus Dimerization Dose-Response Relationship, Drug Follicle Stimulating Hormone / metabolism Genetic Techniques Humans Inhibin-beta Subunits / analysis* Inhibin-beta Subunits / physiology* Inhibins / chemistry K562 Cells Liver / metabolism Rats Recombinant Proteins / analysis* Recombinant Proteins / chemistry |
| IF | 1.735 |
| Times Cited | 11 |
| WOS Category | AGRICULTURE, DAIRY & ANIMAL SCIENCE REPRODUCTIVE BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Wikipedia |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hep G2 |