RRC ID 2401
Author Ogonuki N, Mochida K, Miki H, Inoue K, Fray M, Iwaki T, Moriwaki K, Obata Y, Morozumi K, Yanagimachi R, Ogura A.
Title Spermatozoa and spermatids retrieved from frozen reproductive organs or frozen whole bodies of male mice can produce normal offspring.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Cryopreservation of male germ cells is a strategy to conserve animal species and strains of animals valuable to biomedical research. We tested whether mouse male germ cells could be cryopreserved without cryoprotection by simply freezing epididymides, testes, or whole bodies. The reproductive organs were isolated from killed mice and frozen for 1 week to 1 year at -80 degrees C before spermatozoa and spermatids were collected and injected into mature oocytes. Normal pups were born irrespective of strains tested (ICR and C57BL/6). Epididymides and testes frozen and transported internationally to another laboratory by air could produce pups of inbred C57BL/6 mice. Testicular spermatozoa retrieved from the bodies of male mice (BALB/c nude and C3H/He strains) that had been kept frozen (-20 degrees C) for 15 years could also produce normal offspring by microinsemination. Thus, freezing of either male reproductive organs or whole bodies is the simplest way to preserve male germ cells. Restoration of extinct species could be possible if male individuals are found in permafrost.
Volume 103(35)
Pages 13098-103
Published 2006-8-29
DOI 10.1073/pnas.0605755103
PII 0605755103
PMID 16920794
PMC PMC1550775
MeSH Animals Cryopreservation* Embryo, Mammalian / embryology Epididymis / metabolism Female Fertilization in Vitro Freezing Male Mice Mice, Inbred BALB C Mice, Inbred C57BL Oocytes / metabolism Reproduction / physiology* Specimen Handling Spermatids / cytology Spermatids / physiology* Temperature Testis / cytology Time Factors
IF 9.504
Times Cited 51
WOS Category REPRODUCTIVE BIOLOGY
Resource
Mice