論文 - 詳細
| RRC ID | 2405 |
|---|---|
| 著者 | Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM. |
| タイトル | Detection of autophagy in tissue by standard immunohistochemistry: possibilities and limitations. |
| ジャーナル | Autophagy |
| Abstract |
Transmission electron microscopy (TEM) is currently the standard method to monitor autophagy in tissue. Because TEM is labor intensive, we recently questioned whether marker proteins could be found for unambiguous detection of autophagy in tissue using standard immunohistochemical techniques. Our findings indicated that the identification of autophagy-specific biomarkers for tissue is highly compromised due to lack of differential gene expression. In this respect, TEM remains an indispensable technique for evaluation of autophagy in situ. Nevertheless, immunohistochemical staining of microtubule-associated protein 1 light chain 3 (LC3) appeared to be a valuable technique to detect autophagosome formation in tissue but only when this protein is overexpressed, e.g., in GFP-LC3 transgenic animals. Furthermore, demonstration of granular cytoplasmic ubiquitin inclusions by immunohistochemistry may be an attractive technique to measure autophagic cell degeneration in some human pathologies such as neurodegenerative diseases, heart failure and atherosclerosis. |
| 巻・号 | 2(1) |
| ページ | 55-7 |
| 公開日 | 2006-1-1 |
| DOI | 10.4161/auto.2217 |
| PII | 2217 |
| PMID | 16874065 |
| MeSH | Animals Autophagy* Biomarkers / analysis* Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Humans Immunohistochemistry / methods* Mice Mice, Transgenic Microtubule-Associated Proteins / analysis Microtubule-Associated Proteins / genetics Phagosomes / chemistry Phagosomes / metabolism Sensitivity and Specificity Ubiquitin / analysis |
| IF | 9.77 |
| 引用数 | 57 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |