RRC ID 2405
Author Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM.
Title Detection of autophagy in tissue by standard immunohistochemistry: possibilities and limitations.
Journal Autophagy
Abstract Transmission electron microscopy (TEM) is currently the standard method to monitor autophagy in tissue. Because TEM is labor intensive, we recently questioned whether marker proteins could be found for unambiguous detection of autophagy in tissue using standard immunohistochemical techniques. Our findings indicated that the identification of autophagy-specific biomarkers for tissue is highly compromised due to lack of differential gene expression. In this respect, TEM remains an indispensable technique for evaluation of autophagy in situ. Nevertheless, immunohistochemical staining of microtubule-associated protein 1 light chain 3 (LC3) appeared to be a valuable technique to detect autophagosome formation in tissue but only when this protein is overexpressed, e.g., in GFP-LC3 transgenic animals. Furthermore, demonstration of granular cytoplasmic ubiquitin inclusions by immunohistochemistry may be an attractive technique to measure autophagic cell degeneration in some human pathologies such as neurodegenerative diseases, heart failure and atherosclerosis.
Volume 2(1)
Pages 55-7
Published 2006-1
PII 2217
PMID 16874065
MeSH Animals Autophagy* Biomarkers / analysis* Green Fluorescent Proteins / analysis Green Fluorescent Proteins / genetics Humans Immunohistochemistry / methods* Mice Mice, Transgenic Microtubule-Associated Proteins / analysis Microtubule-Associated Proteins / genetics Phagosomes / chemistry Phagosomes / metabolism Sensitivity and Specificity Ubiquitin / analysis
IF 11.1
Times Cited 55