Reference - Detail
| RRC ID | 2427 |
|---|---|
| Author | Tada A, Kato H, Hasegawa S. |
| Title | Antagonistic effect of EGF against TGF beta1 on transformed phenotype and tropomyosin expression of human lung carcinoma A549 cells. |
| Journal | Oncol Rep |
| Abstract |
TGF beta1 suppressed the colony forming ability of human lung carcinoma cell line, A549 cells in a dose-dependent manner. Accompanying these changes, the levels of the expression of high-molecular weight tropomyosin (TM) isoforms, especially TM1 and TM2, were significantly increased in the TGF beta1-treated A549 cells in a dose-dependent manner. When A549 cells were treated with TGF beta1 (2.5 pM) and EGF (1 nM) simultaneously, anchorage-independent growth of A549 cells was restored. In these cells, the expression of high-molecular weight TM decreased. The results suggest that the levels of the expression of high-molecular weight TM may be involved in antagonistic effects of EGF and TGF beta1 on the anchorage-independent growth of A549 cells. |
| Volume | 7(6) |
| Pages | 1323-6 |
| Published | 2000-1-1 |
| DOI | 10.3892/or.7.6.1323 |
| PMID | 11032937 |
| MeSH | Cell Adhesion / drug effects Cell Division / drug effects Cell Transformation, Neoplastic / drug effects* Dose-Response Relationship, Drug Drug Interactions Epidermal Growth Factor / pharmacology* Humans Lung Neoplasms / metabolism* Lung Neoplasms / pathology* Neoplastic Stem Cells / drug effects Phenotype Protein Isoforms Transforming Growth Factor beta / antagonists & inhibitors* Transforming Growth Factor beta / pharmacology Transforming Growth Factor beta1 Tropomyosin / biosynthesis* Tumor Cells, Cultured / drug effects |
| IF | 3.417 |
| Resource | |
| Human and Animal Cells | A549(RCB0098) |