RRC ID 2436
Author Ueno M, Matsumura M, Watanabe K, Nakamura T, Osakada F, Takahashi M, Kawasaki H, Kinoshita S, Sasai Y.
Title Neural conversion of ES cells by an inductive activity on human amniotic membrane matrix.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Here we report a human-derived material with potent inductive activity that selectively converts ES cells into neural tissues. Both mouse and human ES cells efficiently differentiate into neural precursors when cultured on the matrix components of the human amniotic membrane in serum-free medium [amniotic membrane matrix-based ES cell differentiation (AMED)]. AMED-induced neural tissues have regional characteristics (brainstem) similar to those induced by coculture with mouse PA6 stromal cells [a common method called stromal cell-derived inducing activity (SDIA) culture]. Like the SDIA culture, the AMED system is applicable to the in vitro generation of various CNS tissues, including dopaminergic neurons, motor neurons, and retinal pigment epithelium. In contrast to the SDIA method, which uses animal cells, the AMED culture uses a noncellular inductive material derived from an easily available human tissue; therefore, AMED should provide a more suitable and versatile system for generating a variety of neural tissues for clinical applications.
Volume 103(25)
Pages 9554-9
Published 2006-6-20
DOI 10.1073/pnas.0600104103
PII 0600104103
PMID 16766664
PMC PMC1480445
MeSH Amnion / cytology* Amnion / metabolism Animals Cell Differentiation* Cells, Cultured Coculture Techniques Dopamine / metabolism Extracellular Matrix / metabolism* Eye / cytology Humans Mice Neurons / cytology* Neurons / metabolism Stem Cells / cytology* Stem Cells / metabolism Tissue Culture Techniques
IF 9.504
Times Cited 55
Human embryonic stem cells KhES-1