論文 - 詳細
| RRC ID | 2444 |
|---|---|
| 著者 | Sassa T, Aizawa H, Okamoto H. |
| タイトル | Visualization of two distinct classes of neurons by gad2 and zic1 promoter/enhancer elements in the dorsal hindbrain of developing zebrafish reveals neuronal connectivity related to the auditory and lateral line systems. |
| ジャーナル | Dev Dyn |
| Abstract |
The dorsal hindbrain includes distinct classes of neurons for processing various sensory stimuli, but the developmental aspects of these neurons remain largely unknown. We identify here two distinct classes of neurons in the dorsal hindbrain of developing zebrafish: (1) neurons that express the inhibitory neuronal marker Gad1/2, and (2) neurons that express the zn-5 antigen and Lhx2/9 and require the basic helix-loop-helix transcription factor Atoh1a for development. Neurons were traced to their axon terminals by expressing green fluorescent protein using the Gal4VP16-UAS (UAS, upstream activating sequences) system in combination with the promoter/enhancer regions of gad2 for the Gad1/2(+) neurons and zic1 for the zn-5(+)Lhx2/9(+) neurons. The Gad1/2(+) neurons projected to the contralateral hindbrain, while the zn-5(+)Lhx2/9(+) neurons projected to the contralateral midbrain torus semicircularis, suggesting a role in auditory and lateral line sensory processing. Comparison of these projections with those from the cochlear nuclei to the inferior colliculus in mammals suggests similarities across vertebrate species. |
| 巻・号 | 236(3) |
| ページ | 706-18 |
| 公開日 | 2007-3-1 |
| DOI | 10.1002/dvdy.21084 |
| PMID | 17279576 |
| MeSH | Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Embryo, Nonmammalian / metabolism Enhancer Elements, Genetic / genetics Gene Expression Regulation, Developmental Glutamate Decarboxylase / genetics* Glutamate Decarboxylase / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Immunohistochemistry In Situ Hybridization Lateral Line System / cytology Lateral Line System / embryology Lateral Line System / metabolism* Microscopy, Fluorescence Neurons / cytology Neurons / metabolism* Promoter Regions, Genetic / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Rhombencephalon / cytology Rhombencephalon / embryology Rhombencephalon / metabolism* Transcription Factors / genetics* Transcription Factors / metabolism Zebrafish / embryology Zebrafish / genetics* Zebrafish / metabolism Zebrafish Proteins / genetics* Zebrafish Proteins / metabolism |
| IF | 3.275 |
| 引用数 | 17 |
| WOS 分野 | ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ゼブラフィッシュ | RIKEN WT |