論文 - 詳細
| RRC ID | 2464 |
|---|---|
| 著者 | Flanagan-Steet H, Fox MA, Meyer D, Sanes JR. |
| タイトル | Neuromuscular synapses can form in vivo by incorporation of initially aneural postsynaptic specializations. |
| ジャーナル | Development |
| Abstract |
Synapse formation requires the coordination of pre- and postsynaptic differentiation. An unresolved question is which steps in the process require interactions between pre- and postsynaptic cells, and which proceed cell-autonomously. One current model is that factors released from presynaptic axons organize postsynaptic differentiation directly beneath the nerve terminal. Here, we used neuromuscular junctions (NMJs) of the zebrafish primary motor system to test this model. Clusters of neurotransmitter (acetylcholine) receptors (AChRs) formed in the central region of the myotome, destined to be synapse-rich, before axons extended and even when axon extension was prevented. Time-lapse imaging revealed that pre-existing clusters on early-born slow (adaxial) muscle fibers were incorporated into NMJs as axons advanced. Axons were, however, required for the subsequent remodeling and selective stabilization of synaptic clusters that precisely appose post- to presynaptic elements. Thus, motor axons are dispensable for the initial stages of postsynaptic differentiation but are required for later stages. Moreover, many AChR clusters on later-born fast muscle fibers formed at sites that had already been contacted by axons, suggesting heterogeneity in the signaling mechanisms leading to synapse formation by a single axon. |
| 巻・号 | 132(20) |
| ページ | 4471-81 |
| 公開日 | 2005-10-1 |
| DOI | 10.1242/dev.02044 |
| PII | dev.02044 |
| PMID | 16162647 |
| MeSH | Animals Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Mice Mice, Transgenic Motor Neurons / cytology Motor Neurons / metabolism Muscles / cytology* Muscles / embryology Muscles / metabolism Neurites Neuromuscular Junction / cytology* Neuromuscular Junction / embryology Neuromuscular Junction / genetics Neuromuscular Junction / metabolism Receptors, Cholinergic / metabolism Zebrafish* / embryology Zebrafish* / genetics Zebrafish* / metabolism |
| IF | 5.611 |
| 引用数 | 206 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | rw0 |