論文 - 詳細
| RRC ID | 2474 |
|---|---|
| 著者 | Köster RW, Fraser SE. |
| タイトル | Time-lapse microscopy of brain development. |
| ジャーナル | Methods Cell Biol |
| Abstract |
Zebrafish embryos represent an ideal vertebrate model organism for noninvasive intravital imaging because of their optical clarity, external embryogenesis, and fast development. Many different labeling techniques have been adopted from other model organisms or newly developed to address a wealth of different developmental questions directly inside the living organism. The parallel advancements in the field of optical imaging let us now observe dynamic processes at the cellular and subcellular resolution. Combined with the repertoire of available surgical and genetic manipulations, zebrafish embryos provide the powerful and almost unique possibility to observe the interplay of molecular signals with cellular, morphological, and behavioral changes directly within a living and developing vertebrate organism. A bright future for zebrafish is yet to come, let there be light. |
| 巻・号 | 76 |
| ページ | 207-35 |
| 公開日 | 2004-1-1 |
| DOI | 10.1016/s0091-679x(04)76011-2 |
| PMID | 15602878 |
| MeSH | Animals Animals, Genetically Modified Brain / embryology* Fluorescent Dyes / chemistry Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Histocytological Preparation Techniques / methods Image Processing, Computer-Assisted / methods Imaging, Three-Dimensional / methods Luminescent Proteins / genetics Luminescent Proteins / metabolism Methylcellulose / chemistry Microscopy, Confocal / instrumentation Microscopy, Confocal / methods Microscopy, Fluorescence / instrumentation Microscopy, Fluorescence / methods Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sepharose / chemistry Staining and Labeling / methods Zebrafish / embryology* Zebrafish / genetics |
| IF | 1.441 |
| 引用数 | 17 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ゼブラフィッシュ | NA |