RRC ID 2491
Author Zou J, Beermann F, Wang J, Kawakami K, Wei X.
Title The Fugu tyrp1 promoter directs specific GFP expression in zebrafish: tools to study the RPE and the neural crest-derived melanophores.
Journal Pigment Cell Res
Abstract In vertebrates, pigment cells account for a small percentage of the total cell population and they intermingle with other cell types. This makes it difficult to isolate them for analyzes of their functions in the context of development. To alleviate such difficulty, we generated two stable transgenic zebrafish lines (pt101 and pt102) that express green fluorescent protein (GFP) in melanophores under the control of the 1 kb Fugu tyrp1 promoter. In pt101, GFP is expressed in both retinal pigment epithelium (RPE) cells and the neural crest-derived melanophores (NCDM), whereas in pt102, GFP is predominately expressed in the NCDM. Our results indicate that the Fugu tyrp1 promoter can direct transgene expression in a cell-type-specific manner in zebrafish. In addition, our findings provide evidence supporting differential regulations of melanin-synthesizing genes in RPE cells and the NCDM in zebrafish. Utilizing the varying GFP expression levels in these fish, we have isolated melanophores via flow cytometry and revealed the capability of sorting the NCDM from RPE cells as well. Thus, these transgenic lines are useful tools to study melanophores in zebrafish.
Volume 19(6)
Pages 615-27
Published 2006-12-1
DOI 10.1111/j.1600-0749.2006.00349.x
PMID 17083488
PMC PMC2920493
MeSH Animals Animals, Genetically Modified Cell Differentiation / genetics Cell Line Cell Lineage / genetics Embryology / methods Female Gene Expression Regulation, Developmental / physiology Green Fluorescent Proteins / genetics* Male Melanophores / cytology Melanophores / metabolism* Molecular Biology / methods Neural Crest / cytology Neural Crest / embryology* Neural Crest / metabolism Oxidoreductases / genetics* Pigment Epithelium of Eye / cytology Pigment Epithelium of Eye / embryology* Pigment Epithelium of Eye / metabolism Promoter Regions, Genetic / genetics* Takifugu Transgenes / genetics Zebrafish
Times Cited 25
Zebrafish Reference