RRC ID 2519
Author Egawa C, Kobayashi F, Ishibashi M, Nakamura T, Nakamura C, Takumi S.
Title Differential regulation of transcript accumulation and alternative splicing of a DREB2 homolog under abiotic stress conditions in common wheat.
Journal Genes Genet Syst
Abstract A number of cold responsive (Cor)/late embryogenesis abundant (Lea) genes are induced by both low temperature (LT) and dehydration. To understand the molecular basis of cold acclimation and its relationship with drought stress response in wheat seedlings, we isolated a DREB2 homolog Wdreb2, which is the candidate gene for a transcription factor of the Cor/Lea genes. The Wdreb2 expression was activated by cold, drought, salt and exogenous ABA treatment. Detailed expression studies of Wdreb2 indicated the involvement of two distinct pathways of its activation, a drought and salt stress-responsive pathway and a cold-responsive pathway. The transient expression analysis showed that the Wrab19 expression was directly activated by the WDREB2 transcription factor in wheat cells. Three transcript forms of Wdreb2 (Wdreb2alpha, Wdreb2beta and Wdreb2gamma) were produced through alternative splicing. Under drought and salt stress conditions, the amount of the Wdreb2beta form remained fairly constant during 24-hour treatment, while those of the Wdreb2alpha and Wdreb2gamma forms showed transient increases. On the other hand, the LT treatment resulted in increased transcript levels of all three forms of Wdreb2. Thus, under the LT and drought/salt stress conditions the amount of the WDREB2 transcription factors in wheat is differentially controlled by the level of transcription and alternative splicing.
Volume 81(2)
Pages 77-91
Published 2006-4-1
DOI 10.1266/ggs.81.77
PII JST.JSTAGE/ggs/81.77
PMID 16755132
MeSH Abscisic Acid / pharmacology Acclimatization* Alternative Splicing* Amino Acid Sequence Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Chromosome Mapping Chromosomes, Plant / metabolism Cold Temperature / adverse effects Dehydration / metabolism Gene Expression Regulation* Molecular Sequence Data Oryza / metabolism Phylogeny Plant Proteins / genetics Plant Proteins / metabolism Sequence Homology, Amino Acid Transcription Factors / genetics Transcription Factors / metabolism* Transcriptional Activation Triticum / genetics Triticum / metabolism*
IF 0.917
Times Cited 154
Wheat CS LPGKU2272 nulli-tetrasomic series of CS LPGKU0022~ 24?