| RRC ID |
2534
|
| Author |
Haramoto Y, Takahashi S, Asashima M.
|
| Title |
Two distinct domains in pro-region of Nodal-related 3 are essential for BMP inhibition.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The transforming growth factor-beta (TGF-beta) superfamily member, Xenopus nodal-related 3 (Xnr3), induces neural tissues through inhibition of bone morphogenetic proteins (BMPs). We recently identified an inhibitory mechanism in which the pro-region of Xenopus tropicalis nodal-related 3 (Xtnr3) physically interacts with BMP ligands. Here, we show that disulfide-linked heterodimerization does not contribute to BMP inhibition by Xtnr3 and that the Xtnr3 mature region, overexpression of which can induce the same phenotype as full-length Xtnr3, does not inhibit BMP signaling. Furthermore, we find that the BMP-inhibitory domains of Xtnr3 are separately located in the N- and C-terminal regions of the pro-region. These results indicate the pro-region of Nodal-related 3 is both necessary and sufficient for its BMP inhibition.
|
| Volume |
346(2)
|
| Pages |
470-8
|
| Published |
2006-7-28
|
| DOI |
10.1016/j.bbrc.2006.05.121
|
| PII |
S0006-291X(06)01192-2
|
| PMID |
16762322
|
| MeSH |
Animals
Bone Morphogenetic Proteins / antagonists & inhibitors*
Dimerization
Embryo, Nonmammalian / metabolism
In Vitro Techniques
Mutation
Protein Binding
Protein Structure, Tertiary
Signal Transduction
Transforming Growth Factor beta / biosynthesis
Transforming Growth Factor beta / genetics
Transforming Growth Factor beta / physiology*
Xenopus Proteins / biosynthesis
Xenopus Proteins / genetics
Xenopus Proteins / physiology*
Xenopus laevis
|
| IF |
2.985
|
| Times Cited |
8
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Clawed frogs / Newts |
|
