論文 - 詳細
| RRC ID | 25667 |
|---|---|
| 著者 | Chono M, Matsunaka H, Seki M, Fujita M, Kiribuchi-Otobe C, Oda S, Kojima H, Kobayashi D, Kawakami N. |
| タイトル | Isolation of a wheat (Triticum aestivum L.) mutant in ABA 8'-hydroxylase gene: effect of reduced ABA catabolism on germination inhibition under field condition. |
| ジャーナル | Breed Sci |
| Abstract |
Pre-harvest sprouting, the germination of mature seeds on the mother plant under moist condition, is a serious problem in cereals. To investigate the effect of reduced abscisic acid (ABA) catabolism on germination in hexaploid wheat (Triticum aestivum L.), we cloned the wheat ABA 8'-hydroxyase gene which was highly expressed during seed development (TaABA8'OH1) and screened for mutations that lead to reduced ABA catabolism. In a screen for natural variation, one insertion mutation in exon 5 of TaABA8'OH1 on the D genome (TaABA8'OH1-D) was identified in Japanese cultivars including 'Tamaizumi'. However, a single mutation in TaABA8'OH1-D had no clear effect on germination inhibition in double haploid lines. In a screen for a mutation, one deletion mutant lacking the entire TaABA8'OH1 on the A genome (TaABA8'OH1-A), TM1833, was identified from gamma-ray irradiation lines of 'Tamaizumi'. TM1833 (a double mutant in TaABA8'OH1-A and TaABA8'OH1-D) showed lower TaABA8'OH1 expression, higher ABA content in embryos during seed development under field condition and lower germination than those in 'Tamaizumi' (a single mutant in TaABA8'OH1-D). These results indicate that reduced ABA catabolism through mutations in TaABA8'OH1 may be effective in germination inhibition in field-grown wheat. |
| 巻・号 | 63(1) |
| ページ | 104-15 |
| 公開日 | 2013-3-1 |
| DOI | 10.1270/jsbbs.63.104 |
| PII | 63_104 |
| PMID | 23641187 |
| PMC | PMC3621436 |
| IF | 1.865 |
| 引用数 | 25 |
| WOS 分野 | AGRONOMY PLANT SCIENCES |
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| 各媒体での言及数の合計 | 0 |
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