RRC ID 25670
Author Matsuda R, Iehisa JC, Takumi S.
Title Application of real-time PCR-based SNP detection for mapping of Net2, a causal D-genome gene for hybrid necrosis in interspecific crosses between tetraploid wheat and Aegilops tauschii.
Journal Genes Genet. Syst.
Abstract Available information on genetically assigned molecular markers is not sufficient for efficient construction of a high-density linkage map in wheat. Here, we report on application of high resolution melting (HRM) analysis using a real-time PCR apparatus to develop single nucleotide polymorphism (SNP) markers linked to a hybrid necrosis gene, Net2, located on wheat chromosome 2D. Based on genomic information on barley chromosome 2H and wheat expressed sequence tag libraries, we selected wheat cDNA sequences presumed to be located near the Net2 chromosomal region, and then found SNPs between the parental Ae. tauschii accessions of the synthetic wheat mapping population. HRM analysis of the PCR products from F(2) individuals' DNA enabled us to assign 44.4% of the SNP-representing cDNAs to chromosome 2D despite the presence of the A and B genomes. In addition, the designed SNP markers were assigned to chromosome 2D of Ae. tauschii. The order of the assigned SNP markers in synthetic hexaploid wheat was confirmed by comparison with the markers in barley and Ae. tauschii. Thus, the SNP-genotyping method based on HRM analysis is a useful tool for development of molecular markers at target loci in wheat.
Volume 87(2)
Pages 137-43
Published 2012
PII DN/JST.JSTAGE/ggs/87.137
PMID 22820387
MeSH Alleles Chromosome Mapping / methods Chromosomes, Plant / genetics Crosses, Genetic DNA, Plant / genetics DNA, Plant / isolation & purification Expressed Sequence Tags Genes, Plant* Genetic Linkage Genetic Loci Genetic Markers Hordeum / genetics Plant Proteins / genetics* Plant Proteins / metabolism Poaceae / genetics* Poaceae / growth & development Polymorphism, Single Nucleotide* Real-Time Polymerase Chain Reaction / methods* Sequence Analysis, DNA Tetraploidy Triticum / genetics* Triticum / growth & development
IF 0.913
Times Cited 10
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Wheat KU-2075 KU-2025