| RRC ID |
25671
|
| Author |
Koyama K, Hatano H, Nakamura J, Takumi S.
|
| Title |
Characterization of three VERNALIZATION INSENSITIVE3-like (VIL) homologs in wild wheat, Aegilops tauschii Coss.
|
| Journal |
Hereditas
|
| Abstract |
Control of flowering time is an adaptive trait of plants for different growth habitats. A vernalization requirement is a major genetic component determining wheat flowering time. Arabidopsis VERNALIZATION INSENSITIVE3 (VIN3) and VIN3-like 1 (VIL1) play critical roles in the vernalization pathway of flowering, and three wheat VIL homologs are upregulated by vernalization in einkorn wheat. To study the relationship between vernalization and wheat VIL homologs in Aegilops tauschii, the D-genome progenitor of common wheat, we isolated three cDNAs orthologous to the einkorn wheat VIL genes. The three Ae. tauschii VIL genes showed many single nucleotide polymorphisms including non-synonymous substitutions relative to the einkorn orthologs. In addition, high rates of non-synonymous and synonymous substitutions were revealed by intraspecific variation analysis of the AetVIL sequences, suggesting adaptive evolution at the AetVIL loci. Quantitative RT-PCR analysis was conducted to examine the time course of expression of the VIL genes during vernalization. Of the three AetVIL genes, AetVIL2 was upregulated after one week of low-temperature treatment, and its expression pattern was distinct for winter and spring habit accessions. These observations strongly suggest that AetVIL2 is associated with the vernalization-responsive pathway in Ae. tauschii.
|
| Volume |
149(2)
|
| Pages |
62-71
|
| Published |
2012-4-1
|
| DOI |
10.1111/j.1601-5223.2012.02255.x
|
| PMID |
22568701
|
| MeSH |
Adaptation, Physiological / genetics*
Amino Acid Sequence
Arabidopsis / genetics
Arabidopsis Proteins / genetics*
Cold Temperature
DNA, Complementary / analysis
Flowers / physiology
Gene Expression Regulation, Plant*
Genes, Plant*
Genetic Loci
Homeodomain Proteins / genetics*
Molecular Sequence Data
Phylogeny
Polymorphism, Single Nucleotide / genetics*
Protein Isoforms / genetics
Seasons
Sequence Homology, Amino Acid
Triticum / genetics*
|
| IF |
2.412
|
| Times Cited |
9
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Wheat |
KU-2039
KU-2050
KU-2069
KU-2810 |
