RRC ID |
25673
|
Author |
Takenouchi Y, Nakajima H, Kanamaru K, Takumi S.
|
Title |
Characterization of three homoeologous cDNAs encoding chloroplast-targeted aminolevulinic acid dehydratase in common wheat.
|
Journal |
J Integr Plant Biol
|
Abstract |
In the tetrapyrrole biosynthetic pathway of higher plants, 5-aminolevulinic acid (ALA) is metabolized by ALA dehydratase (ALAD). Here, we isolated ALAD1 cDNA from common wheat (Triticum aestivum L.) and its diploid progenitors, and produced transgenic tobacco plants expressing the wheat ALAD1 gene. The ALAD1 genes were highly conserved among wheat relatives, and three homoeologous loci of wheat ALAD1 (TaALAD1) were equally transcribed in common wheat. A transient expression assay of a TaALAD1-GFP (green fluorescent protein) fusion protein suggested that TaALAD1 is localized in chloroplasts. Overexpression of TaALAD1 in transgenic tobacco resulted in a significant increase in ALAD activity in leaves. Moreover, the transgenic tobacco showed vigorous growth and increased survival rate on medium containing ALA at herbicidal concentrations. These results indicate that wheat ALAD1 has catalytic activity in metabolizing ALA in plastids, and that ectopic expression of TaALAD1 in transgenic plants increases their tolerance to ALA application at high concentrations.
|
Volume |
53(12)
|
Pages |
942-50
|
Published |
2011-12-1
|
DOI |
10.1111/j.1744-7909.2011.01083.x
|
PMID |
22044778
|
MeSH |
Aminolevulinic Acid / pharmacology
Base Sequence
China
Chloroplasts / drug effects
Chloroplasts / enzymology*
Chloroplasts / genetics
Cloning, Molecular
Culture Media / pharmacology
DNA, Complementary / genetics*
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Plant / drug effects
Intracellular Space / drug effects
Intracellular Space / enzymology
Molecular Sequence Data
Phylogeny
Plant Proteins / genetics*
Plant Proteins / metabolism
Plants, Genetically Modified
Porphobilinogen Synthase / genetics*
Porphobilinogen Synthase / metabolism
Protein Transport / drug effects
Sequence Homology, Nucleic Acid*
Tobacco / drug effects
Tobacco / genetics
Tobacco / growth & development
Triticum / drug effects
Triticum / enzymology*
Triticum / genetics*
Triticum / growth & development
|
IF |
4.885
|
Times Cited |
0
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
PLANT SCIENCES
|
Resource |
Wheat |
cDNA database |