論文 - 詳細
| RRC ID | 2584 |
|---|---|
| 著者 | Santini JM, Streimann ICA, Hoven RNV. |
| タイトル | Bacillus macyae sp. nov., an arsenate-respiring bacterium isolated from an Australian gold mine. |
| ジャーナル | Int J Syst Evol Microbiol |
| Abstract |
A strictly anaerobic arsenate-respiring bacterium isolated from a gold mine in Bendigo, Victoria, Australia, belonging to the genus Bacillus is described. Cells are Gram-positive, motile rods capable of respiring with arsenate and nitrate as terminal electron acceptors using a variety of substrates, including acetate as the electron donor. Reduction of arsenate to arsenite is catalysed by a membrane-bound arsenate reductase that displays activity over a broad pH range. Synthesis of the enzyme is regulated; maximal activity is obtained when the organism is grown with arsenate as the terminal electron acceptor and no activity is detectable when it is grown with nitrate. Mass of the catalytic subunit was determined to be approximately 87 kDa based on ingel activity stains. The closest phylogenetic relative, based on 16S rRNA gene sequence analysis, is Bacillus arseniciselenatis, but DNA-DNA hybridization experiments clearly show that strain JMM-4(T) represents a novel Bacillus species, for which the name Bacillus macyae sp. nov. is proposed. The type strain is JMM-4(T) (=DSM 16346(T)=JCM 12340(T)). |
| 巻・号 | 54(Pt 6) |
| ページ | 2241-2244 |
| 公開日 | 2004-11-1 |
| DOI | 10.1099/ijs.0.63059-0 |
| PMID | 15545465 |
| MeSH | Acetates / metabolism Anaerobiosis Arsenates / metabolism* Arsenite Transporting ATPases Arsenites / metabolism Australia Bacillus / classification* Bacillus / cytology Bacillus / isolation & purification* Bacillus / metabolism Bacterial Proteins / isolation & purification Bacterial Proteins / metabolism DNA, Bacterial / chemistry DNA, Bacterial / isolation & purification DNA, Ribosomal / chemistry DNA, Ribosomal / isolation & purification Enzyme Induction Enzyme Stability Genes, rRNA Gentian Violet Gold Hydrogen-Ion Concentration Ion Pumps / metabolism Membrane Proteins / metabolism Mining* Molecular Sequence Data Molecular Weight Movement Multienzyme Complexes / metabolism Nitrates / metabolism Nucleic Acid Hybridization Oxidation-Reduction Phenazines Phylogeny Protein Subunits / chemistry RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology* |
| IF | 2.415 |
| 引用数 | 32 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 病原微生物 | JCM 12340? |