| RRC ID |
2662
|
| Author |
Kubota M, Kawahara K, Sekiya K, Uchida T, Hattori Y, Futamata H, Hiraishi A.
|
| Title |
Nocardioides aromaticivorans sp. nov., a dibenzofuran-degrading bacterium isolated from dioxin-polluted environments.
|
| Journal |
Syst Appl Microbiol
|
| Abstract |
Seven strains of dibenzofuran (DF)-degrading bacteria isolated from dioxin-polluted environments were characterized. These isolates were able to grow with dibenzofuran as the sole carbon and energy source. During the growth with dibenzofuran, they produced a soluble yellow metabolite that exhibited a unique pH-dependent shift of absorption maxima. Dibenzo-p-dioxin and biphenyl were also degraded with pigment production. The isolates were strictly aerobic and chemoorganotrophic and had gram-positive, nonmotile, rod-shaped cells. Chemotaxonomic analyses showed that cells contained L,L-diaminopimeric acid in the peptidoglycan, branched-chain fatty acids as major fatty acids, and menaquinone MK-8(H4) as the sole respiratory quinone. The G + C content of the DNA of the isolates ranged from 72.0 to 72.4 mol%. The 16S rRNA gene sequences of the isolates were very similar to each other (> or = 99.8%). The phylogenetic analysis showed that the isolates formed a cluster with species of the genus Nocardioides with Nocardioides simplex and Nocardioides nitrophenolicus as their nearest neighbors. DNA-DNA hybridization studies showed that the isolates showed a hybridization level of less than 55% to any tested species of the genus Nocardioides. Based on these data, Nocardioides aromaticivorans sp. nov. is proposed for the new DF-degrading isolates. The type strain is strain H-1 (IAM 14992, JCM 11674, DSM 15131).
|
| Volume |
28(2)
|
| Pages |
165-74
|
| Published |
2005-3-1
|
| DOI |
10.1016/j.syapm.2004.10.002
|
| PII |
S0723-2020(04)00025-6
|
| PMID |
15830809
|
| MeSH |
Actinomycetales / chemistry
Actinomycetales / classification*
Actinomycetales / genetics
Actinomycetales / isolation & purification
Bacterial Typing Techniques
Benzofurans / metabolism*
DNA, Bacterial / analysis
DNA, Ribosomal / analysis
Dioxins / metabolism*
Genes, rRNA
Geologic Sediments / microbiology*
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Phylogeny
RNA, Ribosomal, 16S / genetics
Rivers / microbiology*
Sequence Analysis, DNA
Water Pollutants, Chemical / metabolism*
|
| IF |
3.224
|
| Times Cited |
40
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
MICROBIOLOGY
|
| Resource |
| Pathogenic microorganisms |
JCM 11674? |
