RRC ID 2673
著者 Liu J, Nakayama T, Hemmi H, Asano Y, Tsuruoka N, Shimomura K, Nishijima M, Nishino T.
タイトル Microbacterium natoriense sp. nov., a novel D-aminoacylase-producing bacterium isolated from soil in Natori, Japan.
ジャーナル Int J Syst Evol Microbiol
Abstract A rod-shaped, Gram-positive bacterium, strain TNJL143-2(T), having N-acyl-D-amino acid amidohydrolase (D-aminoacylase) activity, was isolated from a soil sample from Natori, Japan. It was a non-spore-forming, strictly aerobic bacterium without motility, showing a temperature optimum for growth of 30 degrees C and a pH optimum for growth of 5-7. The 16S rRNA gene sequence of the strain showed the highest similarities to members of the genus Microbacterium, in particular, Microbacterium aerolatum, Microbacterium foliorum and Microbacterium phyllosphaerae. The chemotaxonomic characteristics, including the compositions of cellular menaquinones, cellular fatty acids and cell-wall amino acids, were consistent with those described for the genus Microbacterium. The G+C content of the genomic DNA was determined as 69.1 mol%. DNA-DNA hybridization studies using type strains of M. aerolatum, M. foliorum and M. phyllosphaerae showed only low levels of relatedness (11-12 %). On the basis of these phenotypic and genotypic results, a novel species, Microbacterium natoriense sp. nov., is proposed, with TNJL143-2(T) (=JCM 12611(T)=ATCC BAA-1032(T)) as the type strain.
巻・号 55(Pt 2)
ページ 661-665
公開日 2005-3-1
DOI 10.1099/ijs.0.63265-0
PMID 15774640
MeSH Actinomycetales / classification* Actinomycetales / enzymology Actinomycetales / genetics Actinomycetales / isolation & purification Amidohydrolases / metabolism* Bacterial Typing Techniques DNA, Bacterial / analysis DNA, Ribosomal / analysis Genotype Japan Molecular Sequence Data Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology*
IF 2.415
引用数 13
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 12137? JCM 11569? JCM 11571? JCM 12611?