RRC ID 2686
著者 Nakajima K, Hirota K, Nodasaka Y, Yumoto I.
タイトル Alkalibacterium iburiense sp. nov., an obligate alkaliphile that reduces an indigo dye.
ジャーナル Int J Syst Evol Microbiol
Abstract Three indigo-reducing obligately alkaliphilic strains, M3(T), 41A and 41C, were isolated. The isolates grew at pH 9-12, but not at pH 7-8. They were Gram-positive, facultatively anaerobic, straight rod-shaped strains with peritrichous flagella. The isolates grew in 0-14% (w/v) NaCl, with optimum growth at 3-13%. They grew at temperatures between 10 and 45 degrees C, with optimum growth at around 30-37 degrees C. They did not hydrolyse starch or gelatin. DL-lactate was the major end-product from D-glucose. No quinones could be detected. The peptidoglycan type was A4beta, Orn-d-Asp. The major cellular fatty acids were C(16:0), C(16:1)7c and C(18:1)9c. The DNA G+C content was 42.6-43.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that the isolates belong to the genus Alkalibacterium. DNA-DNA hybridization revealed low similarity (less than 16%) of the isolates with respect to the two closest phylogenetically related strains, Alkalibacterium olivapovliticus and Alkalibacterium psychrotolerans. On the basis of phenotypic and chemotaxonomic characteristics, phylogenetic data and DNA-DNA relatedness, the isolates merit classification as a novel species of the genus Alkalibacterium, for which the name Alkalibacterium iburiense is proposed. The type strain is M3(T) (=JCM 12662(T)=NCIMB 14024(T)).
巻・号 55(Pt 4)
ページ 1525-1530
公開日 2005-7-1
DOI 10.1099/ijs.0.63487-0
PMID 16014476
MeSH Bacterial Typing Techniques Coloring Agents / metabolism* DNA, Ribosomal / analysis Genes, rRNA Gram-Positive Asporogenous Rods / chemistry Gram-Positive Asporogenous Rods / classification* Gram-Positive Asporogenous Rods / genetics Gram-Positive Asporogenous Rods / metabolism Hydrogen-Ion Concentration Indigo Carmine Indoles / metabolism* Molecular Sequence Data Nucleic Acid Hybridization Oxidation-Reduction Phenotype Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Species Specificity
IF 2.415
引用数 43
WOS 分野 MICROBIOLOGY
リソース情報
病原微生物 JCM 12662? JCM 12281?