Reference - Detail
RRC ID | 27113 |
---|---|
Author | Shiraishi H, Tabuse Y. |
Title | The AplI restriction-modification system in an edible cyanobacterium, Arthrospira (Spirulina) platensis NIES-39, recognizes the nucleotide sequence 5'-CTGCAG-3'. |
Journal | Biosci Biotechnol Biochem |
Abstract |
The degradation of foreign DNAs by restriction enzymes in an edible cyanobacterium, Arthrospira platensis, is a potential barrier for gene-transfer experiments in this economically valuable organism. We overproduced in Escherichia coli the proteins involved in a putative restriction-modification system of A. platensis NIES-39. The protein produced from the putative type II restriction enzyme gene NIES39_K04640 exhibited an endonuclease activity that cleaved DNA within the sequence 5'-CTGCAG-3' between the A at the fifth position and the G at the sixth position. We designated this enzyme AplI. The protein from the adjacent gene NIES39_K04650, which encodes a putative DNA (cytosine-5-)-methyltransferase, rendered DNA molecules resistant to AplI by modifying the C at the fourth position (but not the C at the first position) in the recognition sequence. This modification enzyme, M.AplI, should be useful for converting DNA molecules into AplI-resistant forms for use in gene-transfer experiments. A summary of restriction enzymes in various Arthrospira strains is also presented in this paper. |
Volume | 77(4) |
Pages | 782-8 |
Published | 2013-1-1 |
DOI | 10.1271/bbb.120919 |
PII | DN/JST.JSTAGE/bbb/120919 |
PMID | 23563565 |
MeSH | Amino Acid Sequence Base Sequence Binding Sites DNA Restriction Enzymes / chemistry DNA Restriction Enzymes / isolation & purification DNA Restriction Enzymes / metabolism* DNA, Bacterial / genetics* DNA, Bacterial / metabolism* Escherichia coli / genetics Food Microbiology* Protein Binding Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Recombinant Proteins / metabolism* Spirulina / enzymology* Spirulina / genetics Substrate Specificity |
IF | 1.516 |
Times Cited | 6 |
WOS Category | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Algae | NIES-39 |