Reference - Detail
| RRC ID | 27119 |
|---|---|
| Author | Hautala H, Lamminmäki U, Spoof L, Nybom S, Meriluoto J, Vehniäinen M. |
| Title | Quantitative PCR detection and improved sample preparation of microcystin-producing Anabaena, Microcystis and Planktothrix. |
| Journal | Ecotoxicol Environ Saf |
| Abstract |
Blooms of toxic cyanobacteria, associated with illness and mortality in humans and animals, are becoming increasingly common worldwide. The safe use of surface waters for drinking water production and recreation necessitates assessment of toxigenic cyanobacteria. We have developed simple and reliable sample preparation and qPCR methods to detect microcystin-producing strains of three major bloom-forming genera, Anabaena, Microcystis and Planktothrix. The mcyB second thiolation motif, previously not recognized as a potential target for qPCR, was used as a basis for primer and genus-specific probe design. Assay specificity and sensitivity was confirmed with cultured cyanobacterial strains and the effect of different sample preparation methods on quantification was investigated. Sample filtration and cell lysis reduced assay time and resulted in more efficient amplification compared to DNA extraction. Positive correlation (p<0.005) between mcyB copy numbers and microcystin concentrations was observed in environmental samples. The results encourage the use of qPCR in water risk management. |
| Volume | 87 |
| Pages | 49-56 |
| Published | 2013-1-1 |
| DOI | 10.1016/j.ecoenv.2012.10.008 |
| PII | S0147-6513(12)00360-0 |
| PMID | 23122919 |
| MeSH | Anabaena / classification Anabaena / genetics Animals Cyanobacteria / classification Cyanobacteria / genetics DNA, Bacterial / chemistry Environmental Monitoring / methods* Microcystins / metabolism Microcystis / genetics Real-Time Polymerase Chain Reaction* Risk Management / methods |
| IF | 4.872 |
| Times Cited | 19 |
| WOS Category | TOXICOLOGY ENVIRONMENTAL SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Algae | NIES-107 |