RRC ID 27169
Author Matsumoto M, Sasaki Y, Yasuda K, Takai T, Muramatsu M, Yoshimoto T, Nakanishi K.
Title IgG and IgE collaboratively accelerate expulsion of Strongyloides venezuelensis in a primary infection.
Journal Infect. Immun.
Abstract The host deploys a subset of immune responses to expel helminths, which differs depending on the nature of the helminth. Strongyloides venezuelensis, a counterpart of the human pathogen S. stercoralis, naturally infects rodents and has been used as an experimental model. Here we show that induction of immunoglobulin G (IgG) and IgE is a prerequisite for rapid expulsion of S. venezuelensis during a primary infection. Activation-induced cytidine deaminase-deficient (AID(-/-)) mice, which lack the ability to switch IgM to other isotypes, normally developed T-helper 2 (Th2) cells and intestinal mastocytosis after infection with S. venezuelensis. Although AID(-/-) mice expelled Nippostrongylus brasiliensis normally, they required a much longer period to expel S. venezuelensis than wild-type (WT) mice. Adoptive transfers of immune sera from S. venezuelensis-infected but not N. brasiliensis-infected mice restored the ability of AID(-/-) mice to promptly expel S. venezuelensis. Immune serum-derived IgG and IgE induced worm expulsion via Fc γ receptor III (FcγRIII) and Fc ε receptor I (FcεRI), respectively, and a mixture of IgG and IgE showed collaborative effects. Whereas FcγRIII(-/-) mice or FcεRIα(-/-) mice normally could expel S. venezuelensis, FcγRIII(-/-) mice, when their IgE was neutralized by anti-IgE, or FcεRIα(-/-) mice, when their IgG binding to FcγRIII was blocked by anti-FcγRIII, showed a markedly reduced ability to expel S. venezuelensis. These data reveal that IgG and IgE play redundant roles but act in concert to accelerate S. venezuelensis expulsion. Mast cell-deficient mice, even those equipped with immune serum-derived IgG or IgE, failed to expel S. venezuelensis promptly, suggesting that mast cells are cellular targets of IgG and IgE.
Volume 81(7)
Pages 2518-27
Published 2013-7
DOI 10.1128/IAI.00285-13
PII IAI.00285-13
PMID 23630966
PMC PMC3697603
MeSH Animals Cell Proliferation Immunization, Passive Immunoglobulin Class Switching Immunoglobulin E / administration & dosage Immunoglobulin E / immunology* Immunoglobulin G / administration & dosage Immunoglobulin G / immunology* Mast Cells / immunology Mast Cells / parasitology Mice Mice, Inbred C57BL Mice, Knockout Nippostrongylus / immunology Protein Binding Rats Rats, Sprague-Dawley Rats, Wistar Receptors, IgE / immunology Receptors, IgG / immunology Strongylida Infections / immunology Strongyloides / immunology* Strongyloidiasis / immunology* Strongyloidiasis / prevention & control Th2 Cells / immunology
IF 3.256
Times Cited 10
WOS Category INFECTIOUS DISEASES IMMUNOLOGY
Resource
Mice B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc(RBRC00897)