| RRC ID |
27175
|
| 著者 |
Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y, Kon Y.
|
| タイトル |
Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes.
|
| ジャーナル |
Biol Reprod
|
| Abstract |
Claudin 3 is a protein component of the tight junction strands. Tight junctions between adjacent Sertoli cells form the blood-testis barrier (BTB). During spermatogenesis, seminiferous stage-specific expression of claudin 3 is believed to regulate the migration of preleptotene/leptotene spermatocytes across the BTB. Here, we determined the cell types expressing claudin 3 in adult mouse testis and investigated spermatogenesis after testis-specific in vivo knockdown of claudin 3. The results of in situ hybridization revealed that claudin 3 mRNA was predominantly expressed in germ cells near the basal lamina of seminiferous tubules at stages VI-IX. Furthermore, claudin 3 protein was localized not only to the BTB but also to the cell membrane of STRA8-expressing preleptotene/leptotene spermatocytes in the testis of adult ICR.Cg-Tg(Stra8-EGFP)1Ysa/YsaRbrc mice. Although claudin 3 knockdown did not affect BTB integrity, it did cause a partial delay in spermatocyte migration across the BTB. Moreover, claudin 3 knockdown resulted in a prolonged preleptotene phase during spermatogenesis. These data indicate that the seminiferous stage-specific expression and localization of claudin 3 during spermatogenesis regulate the progression of meiosis by promoting germ cell migration across the BTB.
|
| 巻・号 |
89(1)
|
| ページ |
3
|
| 公開日 |
2013-7-1
|
| DOI |
10.1095/biolreprod.113.107847
|
| PII |
biolreprod.113.107847
|
| PMID |
23677978
|
| MeSH |
Adaptor Proteins, Signal Transducing / metabolism
Animals
Blood-Testis Barrier
Cell Movement / physiology
Claudin-3 / biosynthesis
Claudin-3 / genetics*
Female
Gene Knockdown Techniques
Male
Meiosis*
Mice
Mice, Inbred C57BL
Sertoli Cells / metabolism*
Spermatocytes / metabolism*
Spermatogenesis*
|
| IF |
3.322
|
| 引用数 |
16
|
|
WOS 分野
|
REPRODUCTIVE BIOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC02498 |
