論文 - 詳細
| RRC ID | 27175 |
|---|---|
| 著者 | Chihara M, Ikebuchi R, Otsuka S, Ichii O, Hashimoto Y, Suzuki A, Saga Y, Kon Y. |
| タイトル | Mice stage-specific claudin 3 expression regulates progression of meiosis in early stage spermatocytes. |
| ジャーナル | Biol Reprod |
| Abstract |
Claudin 3 is a protein component of the tight junction strands. Tight junctions between adjacent Sertoli cells form the blood-testis barrier (BTB). During spermatogenesis, seminiferous stage-specific expression of claudin 3 is believed to regulate the migration of preleptotene/leptotene spermatocytes across the BTB. Here, we determined the cell types expressing claudin 3 in adult mouse testis and investigated spermatogenesis after testis-specific in vivo knockdown of claudin 3. The results of in situ hybridization revealed that claudin 3 mRNA was predominantly expressed in germ cells near the basal lamina of seminiferous tubules at stages VI-IX. Furthermore, claudin 3 protein was localized not only to the BTB but also to the cell membrane of STRA8-expressing preleptotene/leptotene spermatocytes in the testis of adult ICR.Cg-Tg(Stra8-EGFP)1Ysa/YsaRbrc mice. Although claudin 3 knockdown did not affect BTB integrity, it did cause a partial delay in spermatocyte migration across the BTB. Moreover, claudin 3 knockdown resulted in a prolonged preleptotene phase during spermatogenesis. These data indicate that the seminiferous stage-specific expression and localization of claudin 3 during spermatogenesis regulate the progression of meiosis by promoting germ cell migration across the BTB. |
| 巻・号 | 89(1) |
| ページ | 3 |
| 公開日 | 2013-7-1 |
| DOI | 10.1095/biolreprod.113.107847 |
| PII | biolreprod.113.107847 |
| PMID | 23677978 |
| MeSH | Adaptor Proteins, Signal Transducing / metabolism Animals Blood-Testis Barrier Cell Movement / physiology Claudin-3 / biosynthesis Claudin-3 / genetics* Female Gene Knockdown Techniques Male Meiosis* Mice Mice, Inbred C57BL Sertoli Cells / metabolism* Spermatocytes / metabolism* Spermatogenesis* |
| IF | 3.322 |
| 引用数 | 16 |
| WOS 分野 | REPRODUCTIVE BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC02498 |