Reference - Detail
| RRC ID | 27307 |
|---|---|
| Author | Nishizawa T, Kita S, Maturana AD, Furuya N, Hirata K, Kasuya G, Ogasawara S, Dohmae N, Iwamoto T, Ishitani R, Nureki O. |
| Title | Structural basis for the counter-transport mechanism of a H+/Ca2+ exchanger. |
| Journal | Science |
| Abstract |
Ca(2+)/cation antiporters catalyze the exchange of Ca(2+) with various cations across biological membranes to regulate cytosolic calcium levels. The recently reported structure of a prokaryotic Na(+)/Ca(2+) exchanger (NCX_Mj) revealed its overall architecture in an outward-facing state. Here, we report the crystal structure of a H(+)/Ca(2+) exchanger from Archaeoglobus fulgidus (CAX_Af) in the two representatives of the inward-facing conformation at 2.3 Å resolution. The structures suggested Ca(2+) or H(+) binds to the cation-binding site mutually exclusively. Structural comparison of CAX_Af with NCX_Mj revealed that the first and sixth transmembrane helices alternately create hydrophilic cavities on the intra- and extracellular sides. The structures and functional analyses provide insight into the mechanism of how the inward- to outward-facing state transition is triggered by the Ca(2+) and H(+) binding. |
| Volume | 341(6142) |
| Pages | 168-72 |
| Published | 2013-7-12 |
| DOI | 10.1126/science.1239002 |
| PII | science.1239002 |
| PMID | 23704374 |
| MeSH | Antiporters / chemistry* Antiporters / genetics Antiporters / metabolism Archaeal Proteins / chemistry* Archaeal Proteins / genetics Archaeal Proteins / metabolism Archaeoglobus fulgidus / metabolism* Binding Sites Calcium / chemistry Calcium / metabolism Cation Transport Proteins / chemistry* Cation Transport Proteins / genetics Cation Transport Proteins / metabolism Crystallography, X-Ray Hydrogen / chemistry Hydrogen / metabolism Protein Structure, Secondary Protein Structure, Tertiary |
| IF | 41.846 |
| Times Cited | 47 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| DNA material | Genomic DNA of Archaeoglobus fulgidus JCM 9628T (JGD06275) |
| General Microbes | JCM 9628 |